气相色谱质谱联用鱼油中脂肪酸含量的测定
毕业论文关键词： 鱼油；二十碳五烯酸；二十二碳吹冰烯酸；气相色谱与质谱联用
Determination of fatty acids in fish oil
Abstract: This article analyzes and studies the contents of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in commercial fish oil capsule by combining with gas chromatography and mass spectrometry. The experiment of catalyst concentration, reaction temperature and time are optimized. Due to different derivation methods, the yield of EPA and DHA vary significantly. It gives the optimum ester exchange reaction conditions were as follows: with Sodium methoxide 1.0mol/L as catalyst, temperature 50 ℃, time of 20 minutes. The appropriate GC-MS oven program：keep at 50℃ for 5min, then 50℃/min to 150℃, and 10℃/min to 340℃, stay for 5min. With linoleic acid methyl ester as an external standard for quantitative analysis, we get the content for EPA and DHA (16.8% and 10.5% ), and the sum is between 20%-30%, which is high-quality fish oil. Therefore, this work introduces ways to discern the false fish oil capsule from the genuine by characteristic index of EPA and DHA in fish oil.
Keywords: Fish oil; EPA; DHA; GC-MS
目录
1 前言 1
1.1 EPA 和DHA的研究分析现状 1
1.2 课题的研究意义和目的 3
1.2.1 研究的意义 3
1.2.2 研究的目的 3
2 实验方法和实验原理 4
2.1 原料、试剂和仪器设备 4
2.1.1 实验原料 4
2.1.2 实验试剂 4
2.1.3 实验仪器设备 4
2.2 实验原理 5
2.2.1 气相色谱-质谱联用仪（GC-MS）原理 5
2.2.2 酯交换反应原理 6
2.2.3 溶剂萃取原理 7
2.2.4 酯化反应原理 8
2.2.5 柱色谱原理 8
2.2.6 旋转蒸发原理 9
2.2.7 薄层层析原理 10
3 实验结果分析与讨论 11
3.1 样品及反应试剂准备 11
3.1.1 样品准备 11
3.1.2 反应试剂准备 11
3.2 碱性条件下的酯交换反应 11
3.2.1 碱性条件下的正交实验设计 11
3.2.2 碱性条件下的实验步骤 12
3.3 酸性条件下的正交实验 13