饮用水源地风险企业废水全组分毒性分析评级技术研究
毕业论文关键词：废水全组分毒性实验；大型蚤；羊角月牙藻；斑马鱼
Rating Technology Research of the Whole Effluent Toxicity Analysis on Risk Enterprises of Source of Drinking Water
Abstract：The thesis study on the whole effluents toxicity test on wastewater of a Shanghai textile company. By using three kinds of aquatic organisms, like: Selenastrum capricornutum, young fleas of daphnia magna and zebra fish to evaluate the test substances which is the wastewater after the production of textile. Because of the different toxic endpoints of the three aquatic organisms, they can tell the toxicity of the effluents. On the condition of short-term exposure, the toxic effect on this three kinds of aquatic organisms can prove that the sample of wastewater may be harmful. The three experiments have conducted on the condition of short-term exposure. The results show that in 72 h, water samples have a certain inhibitory effect for the algae to grow; in 48 h, young fleas of daphnia magna have different degrees of toxicity effect on the ability of swimming and within 96 there is no acute toxicity to zebra fish.
Key Words： WET；Daphnia Magna；Selenastrum Capricornutum；Zebra Fish
目录
1 前言 1
1.1 选题意义 1
1.2 文献综述 1
1.2.1生物监测技术 2
1.2.2全废水毒性测试 3
1.2.3大型蚤毒性测试 3
1.2.4藻类毒性测试 4
1.2.5鱼类毒性测试 5
2 仪器与试剂 6
2.1仪器及用品 6
2.1.1 仪器 6
2.1.2 用品 6
2.2 标准试剂 7
2.3 培养基的配置 7
2.3.1 藻类培养基的配置 7
2.3.2 蚤类培养基的配置 8
2.4 样品的来源与性状 10
3 实验内容 11
3.1 藻类生长抑制试验 11
3.1.1 实验目的与原理 11
3.1.1.1 实验目的 11
3.1.1.2 实验原理 11
3.1.2 准备 11
3.1.2.1 培养基 11
3.1.2.1 羊角月牙藻的预培养 11
3.1.3 预实验 13
3.1.4 正式实验 14
3.1.5计算与讨论 14
3.1.5.1 浓度的换算 14
3.1.5.2 生长曲线下的面积比较 14
3.1.5.3 生长率比较 15
3.1.6 血球计数板的使用 15
3.2 鱼类急性毒性试验 16
3.2.1 实验目的与原理 16
3.2.1.1 实验目的 16
3.2.1.2 实验原理 16
3.2.2 准备 16
3.2.2.1 斑马鱼的驯养 16
3.2.2.2 试验用水 16
3.2.2.3 暴露条件 16
3.2.3 预实验 17
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