功能化有机无机复合介孔膜电化学传感器的研制
毕业论文关键词: 生物传感器；肌红蛋白；有机/无机复合介孔膜；化学修饰电极；过氧化氢
Development of functionalized Organic/Inorganic Hybrid Mesoporous Silica Membrane electrochemical sensors
Abstract：In this work, organic/inorganic hybrid mesoporous silica membranes (HMSMs) were rapidly synthesized using P123 as the soft template and PC (polycarbonate membrane) as the hard template by microwave-assisted method combined with solvent extraction. The myoglobin (Mb) was physically adsorded to HMSM by shaking to obtain Mb/HMSM, then the Mb/HMSM was fixed onto a glassy carbon electrode surface via Nafion solution, forming modified electrode Mb/HMSM/GCE. Under optimal conditions, the direct electrochemical behavior on modified electrode displayed a pair of well-defined quasi-reversible redox peaks, with the formal potential of ~0.7 V. Electrode reaction is a surface-controlled process. The electrocatalytic experiments of modified electrode towards hydrogen peroxide showed that, Mb/HMSM/GCE can electrocatalytically reduce hydrogen peroxide, giving a linear response range over 0.5-4.5 mM, catalytic sensitivity of 2.43 μA mM-1 cm-2 and a detection limit of 0.18 mM (S/N = 3). These results show that though the performance of Mb/HMSM/GCE is undesirable, as a novel protein carrier, HMSM is expected to prepare a long-life, low detection limit, and more promising electrochemical biosensor, which will deepen the understanding of the myoglobin-related electron transfer process.
Key words: Biosensors; Myoglobin; Organic/Inorganic Hybrid Mesoporous Silica Membrane; Chemically modified electrode; Hydrogen peroxide
目录
1 绪论 1
1.1 选题研究现状及发展趋势 1
1.1.1 膜材料简介与发展趋势 1
1.1.2 膜材料表征 3
1.1.3 蛋白质简介 4
1.1.4 电化学生物传感器 5
1.2 选题研究目的和意义 6
2 实验部分 8
2.1 实验仪器和试剂 8
2.1.1 仪器 8
2.1.2 试剂 9
2.1.3 试剂的配制 9
2.2 实验内容 11
2.2.1 有机/无机复合介孔膜的微波辅助合成及溶剂萃取 11
2.2.2 Mb/HMSM修饰电极（Mb/HMSM/GCE）的制备和电化学测量 11
2.2.3 实验仪器及装置图 13
3 结果与讨论 16
3.1 合成与材料宏观 16
3.1.1 材料宏观照片 16
3.2 Mb/HMSM修饰电极（Mb/HMSM/GCE）上的肌红蛋白电化学行为 17
3.2.1 Mb/HMSM修饰电极的直接电化学 17
3.2.2 扫描速度对Mb直接电子转移的影响 18
3.2.3 溶液pH值对Mb直接电子转移的影响 19