摘 要：本文研究了从苏北地产麦冬果中提取色素的适宜于工业生产的方法。以麦冬果为原料, 水做为溶剂，采用超声辅助溶剂提取方法,在均匀设计方案下探讨了超声功率、浸提温度、超声提取时间三因素对提取物的吸光度的影响；采用计算机软件Excel对因素及结果进行回归分析，得到了因素和吸光度的数学模型为： 

Y=1。592518419-0。01412345*X1-0。028399191*X2-0。008758311*X3+0。000429811*X1*X2

进而优选出了适宜的工艺条件为超声功率66。1%，浸提温度32。9℃，提取时间10min。用此条件验证，吸光度为0。567，与理论预测值0。572基本一致。超声波辅助均匀设计法提取麦冬果色素是行之有效的方法。93803

毕业论文关键词：麦冬果，色素，超声提取法，均匀设计法

Abstract：In this paper, a method for the suitable industrial extracted pigment from fruit of Ophiopogon japonicus were researched。 The fruit of Ophiopogon japonicus were used as extraction materials and distilled water were used as solvent。 The extraction of the pigment were carried out using combination methods both an ultrasound-assisted distilled water extraction process and uniform design method。 The effect factors on absorbance of pigment, such as extraction temperature, ultrasonic irradiation time and power were tested。 A mathematical model of the effect factors and the absorbance is regressed by using Excel software 2007:

Y=1。592518419-0。01412345*X1-0。028399191*X2-0。008758311*X3+0。000429811*X1*X2

The optimal extraction conditions are solved through equation, that are 66。1% ultrasonic power, the extraction temperature of 32。9℃, ultrasonic irradiation time 10 min and the real extraction yield of total active ingredients is 0。567 and the predicted value 0。572 under these conditions。 Ultrasonic assisted uniform design method to extract pigment from fruit of Ophiopogon japonicus is an effective method。

Keywords：Ophiopogon japonicas, pigment, ultrasonic extraction, uniform design method

目  录

1  前言 4

2 实验部分 5

2。1 仪器、试剂与材料 5

2。1。1 仪器 5

2。1。2 试剂 5

2。1。3实验材料 5

2。2原料的前处理 5

2。3麦冬果实色素的光谱特征 5

2。4 温度、料液比对实验的影响 6

2。4。1 温度对色素的影响 6

2。4。2 料液比的确定 6

2。5 超声辅助均匀设计应用于原料的提取 6

2。5。1 均匀设计优化方案及结果 6

2。5。2 最大吸收波长分析 7

2。5。3回归数据分析 7

2。5。4最优工艺的推导及验证 11

2。5。5本节小结 12

2。6麦冬果中色素成分的存在部位分析 12

2。6。1分离方法 12

2。6。2结果分析 12

2。6。3实验验证 13

2。6。4本节小结 13

结 论