猪屎豆中光萼猪屎豆碱的提取分离及体外杀伤肿瘤细胞的作用
以海南岛产的猪屎豆种子为原料，采用无水乙醇提取法，用石油醚，氯仿多次除杂，最后用混合溶剂重结晶得到有效成分，并用核磁共振氢谱、碳谱以及质谱对其进行结构表征，以及它对人胃癌细胞MGC-803、人肝癌细胞HepG-2、人乳腺癌细胞MCF-7体外增殖影响的研究。结果表明：用核磁共振法证实有效成分为光萼猪屎豆碱，光萼猪屎豆碱对人胃癌细胞MGC-803、人肝癌细胞HepG-2、人乳腺癌细胞MCF-7体外增殖有抑制作用，细胞凋亡较明显，且在测定范围内随浓度增大，抑制作用越强。28231
关键词 猪屎豆 提取 结构表征 对癌细胞抑制作用
毕业论文设计说明书外文摘要
Title Extraction and Separation of Usaramine in Crotalaria and the tumor cell Killing effect in vitro
Abstract
Crotalaria is leguminous crotalaria plants, distributed in the south of china widely. Various types of alkaloids that crotalaria contained have great medicinal value, many of them have been used in clinical treatment.
The raw material is crotalaria seed produced in Hainan Island, extracted it by ethanol method, using petroleum ether, chloroform to remove its impurity repeatedly, and finally, using mixed solvent recrystallized to obtain effective components. And characterized its structure by NMR hydrogen spectrum, carbon spectrum and mass spectrum, as well as the study on the proliferation effect of human gastric cancer cell MGC-803, human hepatoma cell HepG-2, and human breast cancer cell MCF-7. The results show that the active ingredient that has been confirmed by nuclear magnetic resonance spectroscopy is porella crotalaria base, porella crotalaria base has inhibition on the proliferation of human gastric cancer cell MGC-803, human hepatoma cell HepG-2 and human breast cancer cell MCF-7, apoptosis is obvious, and in the measurement range with the increase of concentration, the inhibition is stronger.
Keywords Crotalaria Extract Structural characterization Inhibition of cancer
目 次
1 绪论 1
1．1 猪屎豆的概述 1
1．1．1 猪屎豆的特点 1
1．1．2 猪屎豆属植物中的化学组成 1
1．1．3 猪屎豆中光萼猪屎豆碱 1
1．2 猪屎豆属植物的作用及价值 1
1．2．1 药用价值 1
1．2．2 固氮作用 2
2 猪屎豆中光萼猪屎豆碱的提取分离及结构表征 3
2．1 材料与实验仪器 3
2．1．1 材料 3
2．1．2 主要试剂 3
2．1．2 主要仪器 3
2．2 提取与分离 3
2．2．1 用无水乙醇提取 3
2．2．2 粗提取物的除杂 5
2．2．3 二氯甲烷提取液的处理 5
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