枸杞中原花青素的组成与含量分析
毕业论文关键词：枸杞；含量分析；原花青素；纯化；抗氧化
Analysis on the Composition and Content of Proanthocyanidins in Chinese Wolfberry
Abstract：In this experiment, Ningxia Lycium ruthenicum dry goods (2015 production and 2016 production) as the raw material, we have determined the content of polyphenols, flavonoids, proanthocyanidins and anthocyanin in Lycium ruthenicum. Using the macroporous resin static adsorption experiments, we have determined the optimum resin type of extracting Procyanidins , the crude extract of procyanidins was purified to prepare macroporous resin purified samples. Taking vitamin C as a positive control, analysis the antioxidant activity of proanthocyanidins in Lycium ruthenicum crude extract and AB-8 purified samples ,with DPPH free radical clearance rate, ABTS free radical clearance rate, superoxide anion free radical clearance rate, FRAP value, total reducing power index. The results show that the content of polyphenols, flavonoids, procyanidins, anthocyaninsin 2015 production and 2016 production Ningxia Lycium ruthenicum extracts were abundant, and the content of chemical components in 2016 production Lycium ruthenicum were higher than 2015 production Lycium ruthenicum, and the difference is obvious. Purified with AS-8 resin, the content of proanthocyanidin in Lycium ruthenicum proanthocyanidin extracts increased from 7.6% to 76.8%. Procyanidins extracts and purified substance have good antioxidant activity, of which the proanthocyanidins purified samples at the concentration of 200 ug/mL ,DPPH free radical clearance rate, ASTS free radical clearance rate and superoxide anion free radicals clearance rate were 53.72%, 95.36% and 86.75%, good antioxidant activity and the antioxidant index were higher than that of proanthocyanidin extracts, showed that procyanidins crude extract after purification the antioxidant activity of it were promoted, which were potential of natural plant composition having good antioxidant activity.
Key words: Chinese Wolfberry;Oligomeric Proanthocyanidins; Extract;Antioxidation;Content Analysis
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料 3
1.1 原料 3
1.2 主要试剂3
1.3 主要仪器与设备3
2 实验方法3
2.1 多酚提取和含量测定方法3
2.2 黄酮提取和含量测定方法3
2.3 原花青素提取和含量测定方法4
2.4 花青素提取和含量测定方法4
2.5 大孔树脂纯化黑果枸杞原花青素4
2.5.1 大孔树脂的选择4
2.5.2黑果枸杞原花青素的纯化4
2.6 抗氧化实验方法 5
2.6.1 DPPH自由基5
2.6.2 ABTS自由基5
2.6.3 超氧阴离子自由基5
上一篇：葡萄品质无损评价技术和仪器研发