甾体药物中间体生物转化条件的优化
毕业论文关键词：甾体化合物；植物甾醇；生物转化；4-雄(甾)烯-3,17-二酮（AD）；高效液相色谱法
Opitmization of transformation conditions of steroid drug intermediates
Abstract: The purpose of this experiment is to select different formulation by its mobile phase, an organic solvent and purified solvent to optimize the increase the amount of androst-4-ene-3,17-dione , inhibit the formation of intermediates obtained yield androst-4-ene-3,17-dione, thereby improving the conversion rate. The experiment method is to take the seed through the medium of phytosterols, fermentation medium, extraction, concentration, purification, and centrifugation step was converted to androst-4-ene-3,17-dione. By high-performance liquid chromatography of androst-4-ene-3,17-dione to determine the content, then get the conversion rate by calculation. Taking methanol as the solvent was purified androst-4-ene-3,17-dione in the formulation under conditions Ⅴ conversion was 41.2% and the conversion rate is high in the five kinds of formulations, but did not reach the desired conversion values. Taking ethyl acetate as the solvent was purified androst-4-ene-3,17-dione in the formulation under conditions Ⅴ conversion was 84.3%, the conversion of the formula 5 maximum and reach the ideal conversion values.
Keywords : steroids ; phytosterols ; biotransformation ; Androstenedione (AD); HPLC
目 录
1 研究背景 1
1.1甾体化合物及甾体药物 1
1.2 甾体化合物的微生物转化 2
1.3 植物甾醇及甾醇侧链降解 2
1.3.1 植物甾醇 3
1.3.2 侧链降解产物AD 3
1.4 雄甾-4-烯-3,17-二酮的分析方法 4
1.4.1 紫外分光光度法 4
1.4.2 气相色谱法（GC） 4
1.4.3 高效液相色谱法（HPLC） 5
1.5 本课题的研究意义及研究内容 5
1.5.1研究意义 5
1.5.2 研究内容 5
2 实验材料及方法 6
2.1 实验材料 6
2.1.1 原材料 6
2.1.2 主要试剂 6
2.1.3 主要仪器 7
2.2 实验部分 7
2.2.1 种子培养基的配制 7
2.2.2 发酵培养基的配制 8
2.2.3 种子培养基的接种 8
2.2.4 发酵培养基的接发酵 8
2.2.5 流动相的选择 8
2.2.6 有机溶剂的选取 8
2.2.7 纯化溶剂的选择 8
2.2.7.1 选用乙酸乙酯作为纯化溶剂 8
2.2.7.2 选用甲醇溶液作为纯化溶剂 9
2.2.8 取样 9
2.2.9 雄烯二酮标准曲线的绘制 9
2.2.10 分析检测 9
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