模拟微重力对人外周血T淋巴细胞功能的影响
毕业论文关键词 微重力 旋转细胞培养系统 T淋巴细胞 细胞增殖 白细胞介素-2
毕业设计说明书外文摘要
Title The change of the function of T lymphocyte in human peripheral blood on simulated microgravity condition
Abstract
Some reports show that space flight can have negative effects on the astronauts’ immune system, it leads to bacterial, fungal or viral infections, and increase the risk of autoimmune disease. Space flight also lead to organisms’ immune system change and the latent virus to be activated.The purified T lymphocyte from human peripheral blood is the research object of this article . We studied the comparison of cell vitality and the expression change of IL-2 under different culture conditions including the rotary cell culture system (RCCS) under the simulated microgravity conditions and the horizontal conditions.The method we used to detect the T cell’s vitality was the CCK-8 . And the method to test the IL-2 content was the enzyme-linked immunosorbent assay. The results show that under the microgravity condition, the vitality of T cell and IL-2 expression changed, microgravity conditions significantly reduce the vitality of T cell and inhibit the production of IL - 2 when the ConA’s stimulation exist or not.
Keywords Microgravity Rotary cell culture system T lymphocyte Cell proliferation Interleukin-2
目 次
1 引言 ………………………………………………………………………………………… 3
2 材料和试剂 ………………………………………………………………………………… 6
3 原理和方法 ………………………………………………………………………………… 7
3.1 人外周血淋巴细胞的提取 …………………………………………………………………7
3.2 尼龙毛柱法分离T淋巴细胞 …………………………………………………………… 8