荧光探针法测定硫酸盐阴离子表面活性剂的临界胶束浓度
毕业论文关键词：稳态荧光探针法；胶束聚集数；十二烷基硫酸钠；临界胶束浓度；芘
Probe method for the determination of the aggregation behavior of sulfate anionic surfactant
Abstract：Pyrene as a fluorescence probe, the determination of a series of surfactants under different conditions of K12 after solubilization of pyrene fluorescence spectra of K12 anionic surfactant critical micelle concentration (CMC) test methods. According to the I 338 / I 333 and K12 concentration variation available for K12 critical micelle concentration is 0.8011 × 10-3. At the same time, externally added reagent such as inorganic salts, ethanol, glycerol affect its critical micelle concentration. Pyrene as a fluorescence probe and benzophenone as the quencher, the steady-state fluorescence probe method under different surfactant concentrations were determined micellar aggregation number 64. By the micellar aggregation number and surfactant concentration equation outside Deriving got K12 micellar aggregation number Nm = 21.69+8.51C.
Keywords：Fluorescence Probe Method；Nm；K12；CMC；Pyrene
目录
1 前言 1
1.1 表面活性剂的现状和发展趋势 1
1.2表面活性剂的发展前景 2
1.3临界胶束浓度（CMC）的定义及测定方法 3
1.3.1 最大气泡压力法 3
1.3.2 滴重法 3
1.3.3 表面张力法 3
1.3.4 紫外分光光度法 3
1.3.5 电导法 4
1.3.6 吸附伏安法 4
1.3.7 荧光探针法 4
1.4胶束聚集数(N)的定义及测定方法 5
1.5本课题的研究目的及意义 5
2 实验原理与方法 6
2.1 实验原料与仪器设备 6
2.1.1 实验原料 6
2.1.2 实验仪器与设备 6
2.2 实验步骤 7
2.2.1 芘标准贮备溶液配制 7
2.2.2 K12标准贮备液配制 7
2.2.3 K12标准工作溶液的配制 7
2.2.4 K12-氯化钠标准工作溶液的配制 7
2.2.5 K12-乙醇标准工作溶液的配制 8
2.2.6 K12-丙三醇标准工作液的配制 8
2.2.7 测定胶束聚集数所需溶液的配制 9
2.3 测试方法 9
2.3.1 K12的临界胶束浓度的测定 10
2.3.2胶束聚集数的测定 10
3 实验结果与讨论 11
3.1 K12临界胶束浓度的测定 11
3.1.1不同浓度K12表面活性剂溶液中芘的激发荧光光谱 11
3.1.2 不同浓度K12溶液中芘的发射荧光光谱 11
3.1.3 荧光激发光谱中I338/I333（I1/I2）和荧光发射光谱中I373/I384（I1/I2）随K12浓度的变化曲线 12
3.2 无机盐对K12临界胶束浓度的影响 14
3.3 醇（单元醇）对K12临界胶束浓度的影响 19
3.4 醇（多元醇）对K12临界胶束浓度的影响 25
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