三萜化合物波谱性能的测试+文献综述
毕业论文关键词：白桦脂酸；白桦脂醇；桦木酮酸；经包埋处理的白桦脂酸；纳米BOA-RUB；三萜化合物；红外光谱；紫外光谱；投射电镜
The chemical spectrum test of pentacyclic triterpenoids compounds
Abstract: To determine the total tritepenoidic in pentacyclic triterpenoids compounds using ultraviolet spectrophotometri method.Also,IR spectrum test and Transmission Electron Microscope are required in order to get spectral properties.: total tritepenoidic is determined by spectrometry using ursolic acid as standard and 5%vanillin-glacial acetic and perchloric acid as color-developing agents. Spectrogram of pentacyclic triterpenoids compounds is determined by infrared spectroscopy and transmission Electron Microscope.After these tests,maximum absorption wavelength of betulinic acid is detected at 341nm and the total tritepenoidic is 0.1148%.The infrared spectrograms of betulinic acid, betulin,betulonic acid and betulinic acid after being embedded are obtained. The structure of Nano BOA-BUB is pictured by transmission electron microscope.
KeyWords： Betulinic acid,betulin, betulonic acid,Nano BOA-BUB,three terpene compounds, infrared spectroscopy, ultraviolet spectroscopy,transmission Electron Microscope
目 录
1 本课题国内外研究现状概述 3
1.1 抗肿瘤 4
1.2 抗HIV 4
1.3 抗炎 5
1.4 其它生物活性 5
2本课题的研究目的和意义 6
3紫外分光光度法测定白桦脂酸样品中的总三萜类成分的含量 7
3.1 仪器与试剂 7
3.2 方法与结果 7
3.2.1 对照品溶液制备 7
3.2.2 显色剂溶液的制备 7
3.2.3 波长的选择 8
3.2.4 显色剂使用条件选择 8
3.2.5 高氯酸用量考察 9
3.2.5 水浴温度考察 9
3.2.6 水浴时间考察 10
3.2.7 标准曲线绘制 10
3.2.8 样品含量测定 11
3.2.9 精密度试验 12
3.2.10 稳定性试验 12
4红外及近红外波谱分析技术的基本原理 12
4.1仪器和试剂 13
4.2已知的标准样品红外光谱集建立 13
4.3 三萜化合物红外光谱测试 14
4.4 红外光谱图分析 14
4.4.1 Nano—BeA红外光谱图分析 14
4.4.2白桦脂醇红外光谱图分析 16
4.4.3白桦酯酸的红外光谱分析 16
5.0 三萜化合物透射电镜测试 17
5.1 透射电镜的原理 18
5.2 透射电镜的结构 18
5.3 投射电镜实验方法与结果 19
5.3.1 仪器与试剂 19
5.3.2 仪器操作步骤 19
5.3.4 样品成像记录 21
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