铜萃取有机物影响下氧化亚铁钩端螺旋菌吸附和浸铜行为研究
毕业论文关键词：生物浸铜；溶剂萃取；LIX984N；氧化亚铁钩端螺旋菌；吸附
Abstract
Leptospirillum ferrooxidans were grown on low grade chalcopyrite and organic phase from copper solvent extraction and entrainment of aqueous raffinate were studied by different ways of contamination in the paper to investigate the alterations of the properties of growth, adsorption and leaching by the cell concentration, pH, the solution potential and Fe2+. It was found that an organic phase inhibited the growth of Leptospirillum ferrooxidans , the growth rate of Logarithmic phase and the highest concentration decreased, the time to stabilize period extended. The adsorption rate of 5% LIX984N organic phase polluted bacteria in the chalcopyrite surface increases, which in ore energy grew slower than in ferrous energy. Inhibition of different ways of contamination for the growth and leaching of Leptospirillum ferrooxidans from strong to weak were contaminated medium and contaminated bacteria, contaminated medium, contaminated bacteria and the contral bacteria.
KeyWords：bioleaching；solvent extraction；LIX984N；Leptospirillum ferrooxidans；Adsorption
目 录
1 绪言 1
1.1 研究背景 1
1.2 生物冶金的发展与现状 1
1.3 微生物浸矿机理 2
1.4 浸矿细菌的吸附行为 4
1.4.1 吸附机理 4
1.4.2 关于胞外聚合物（EPS）的研究 6
1.5 铜萃取萃余液夹带行为研究 6
1.6 铜萃取有机相对浸矿细菌的影响 7
1.7 研究的目的、意义和内容 7
1.7.1 研究的目的和意义 7
1.7.2 研究内容 7
2 实验材料与方法 8
2.1 实验材料 8
2.1.1 实验菌种 8
2.1.2 实验仪器与试剂 8
2.1.3 矿样 8
2.1.4 萃取剂 8
2.1.5 稀释剂 9
2.1.6 培养基 9
2.2 实验方法 9
2.2.1 磨矿与洗矿 9
2.2.2 灭菌方法 9
2.2.3 细菌的培养 9
2.2.4 细菌以黄铜矿为能源的驯化 10
2.2.5 萃取有机相不同污染方式的模拟 10
2.2.6 氧化亚铁钩端螺旋菌在黄铜矿表面的吸附 10
2.2.7 氧化亚铁钩端螺旋菌对黄铜矿的浸出 11
2.3 分析方法 11
2.3.1 浸出液pH值和氧化还原电位的测定 11
2.3.2 Fe2+测定 11
2.3.3 细菌浓度分析 12
3 结果与讨论 13
3.1 铜萃取有机物对氧化亚铁钩端螺旋菌生长的影响 13
3.2 铜萃取有机物对氧化亚铁钩端螺旋菌吸附的影响 16
3.3 铜萃取有机物对氧化亚铁钩端螺旋菌浸出黄铜矿的影响 17
上一篇：碱性镀锌工艺研究+文献综述