竹荪美白作用的研究
毕业论文关键词：竹荪；黑色素瘤细胞；定性检测；美白；
Research of bamboo-sun whitening effect
Abstract: In this article, through bamboo-sun mycelium of different concentrations of alcohol sink and supernatant fluid extract respectively with melanoma cells in mice, in does not affect the premise of the activity of cells and to analyze its effect on tyrosinase activity of melanoma cell, in turn, affects the formation of melanin, looking for bamboo-sun has whitening effect of the components. Method is the solution of different concentrations of ethanol extraction bamboo-sun mycelia and fermented liquid, to extract the qualitative inspection. Cultivation of B16 melanoma cells. Determination of bamboo-sun extract on B16 cell proliferation inhibition, and study different extract effect on tyrosinase activity of B16 cells. Results show that the bamboo-sun mycelium water extract of 75% alcohol and sunken under 40% and 80% concentration of the two, the inhibition of proliferation of B16 cells, and has good inhibition of tyrosinase activity.
KeyWords: Bamboo-sun; Melanoma cells. Qualitative detection; Whitening
目录
1 绪论 1
1.1 竹荪特性研究概况 1
1.1.1 降血压、血脂作用 1
1.1.2 抗辐射、调节免疫作用 2
1.1.3 抗肿瘤作用 2
1.1.4 抗氧化作用 2
1.1.5 细胞的凝集作用 2
1.1.6 抑菌作用 2
1.2 定性检测实验的研究概况 3
1.2.1定性分析诠释 3
1.2.2分类 3
1.2.3分析方法 3
1.2.4操作方式 4
1.2.5灵敏度 4
1.2.6干扰 4
1.2.7分析要求 5
1.2.8分析步骤 5
1.3 竹荪美白特性研究概况 6
1.4研究目的和意义 7
1.5 主要研究内容 7
2 实验材料和方法 7
2.1实验材料 7
2.1.1原料与试剂 7
2.1.2 仪器与设备 9
2.2 实验方法 9
2.2.1发酵方法 9
2.2.2竹荪菌丝体上清液与醇沉的分离 9
2.2.3小鼠B16黑色素瘤细胞的培养 10
2.2.4 B16细胞中酪氨酸酶活性实验 10
2.2.5小鼠B16黑色素瘤细胞增殖活性测定（CCK-8法） 10
2.2.6酶标仪的检测 10
2.2.7生物碱的定性检验： 11
2.2.8氨基酸、肽和蛋白质检验： 11
2.2.9有机酸的定性检验： 11
2.2.10酚类化合物和鞣质的定性检验： 11
2.2.11甾体的定性检验： 11
2.2.12黄酮体的定性检验： 12
2.2.13蒽醌类的定性检验： 12
2.2.14糖类的定性检测 12
3 结果与讨论 13
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