摘要:该篇文章主要以芘作为荧光探针，通过荧光光谱仪测定不同浓度含芘PEG-40氢化蓖麻油表面活性剂的激发光谱和发射光谱，从而得出PEG-40氢化蓖麻油非离子表面活性剂临界胶束浓度（CMC）。除此之外，还深究了氯化钠、乙醇及丙三醇这三种物质对非离子表面活性剂临界胶束浓度的影响。由实验得出，无机盐氯化钠的加入会使得PEG-40氢化蓖麻油的CMC值显著减少；当向PEG-40氢化蓖麻油中添加乙醇时，会出现其CMC的值先降低后增大；而丙三醇的加入导致表面活性剂CMC随着其加入量的增加都表现出增大的趋势。根据激发光谱得出的I338/I333和发射光谱测得的I1/I3与不同浓度PEG-40氢化蓖麻油的变化关系，可得到PEG-40氢化蓖麻油非离子表面活性剂临界胶束浓度分别为2.07×10-2 mol/L和1.98×10-2 mol/L。41827

毕业论文关键字:荧光探针；表面活性剂；临界胶束浓度；芘

Fluorescent probe method measure critical micelle concentration of PEG - 40 hydrogenated castor oil

Abstract: This article is mainly to pyrene as a fluorescence probe, using fluorescence spectrometer determined different concentration containing pyrene PEG -40 hydrogenat -ed castor oil surfactant excitation spectrum and emission spectrum, calculated the PEG - 40 hydrogenated castor oil nonionic surfactant critical micelle concentration (CMC).In addition, it also explore the influence of sodium chloride, ethanol and glycerol these three substances to critical micelle concentration of non-ionic surfactant. By experiment, the addition of inorganic salt sodium chloride makes PEG - 40 hydrogenated castor oil of CMC values significantly reduced; When adding ethanol to the PEG - 40 hydrogenated castor oil, it can appear the CMC decreases after increasing; While the addition of glycerol can cause surfactants CMC values are expressed as increasing trend. Accor-  ding to the excitation spectrum of I338 / I333 and emission spectrum measured I1 / I3 with different concentrations of PEG - 40 and the changes of hydrogenated castor oil can get a PEG - 40 hydrogenated castor oil, nonionic surfactant critical micelle concentration were 2.07 x 10-2 mol/L and 1.98 x 10-2 mol/L.

Key words: fluorescent probe; the surfactant; Critical micelle concentration; pyrene

目 录

1 引言 1

1.1 本课题国内外研究现状概述 1

1.1.1 表面活性剂的概述 1

1.1.2 PEG-40氢化蓖麻油的性质和应用 1

1.2 临界胶束浓度（CMC）的定义 2

1.2.1 胶束的形成机制 2

1.2.2 胶束化作用 2

1.3 临界胶束浓度的测定方法 3

1.3.1 表面张力法 3

1.3.2 电导法 3

1.3.3 吸附伏安法 4

1.3.4 超滤法 4

1.3.5 紫外分光光度法 4

1.3.6 染料吸附法 5

1.3.7 溶解度法 5

1.3.8 光散射法[14] 5

1.3.9 荧光探针法 5

1.4 本课题研究的目的和意义 7

2 实验部分 8

2.1 实验原料 8

2.2 实验仪器与设备 8

2.3 实验溶液的配制