摘要：生物能源作为一种再生的清洁能源，是能够替代化石燃料的理想能源。本实验选择水稻和芒草为原料，用NaOH的预处理，分析细胞壁的成分转变对预处理和酶解的产糖效率的影响。

实验测定了秸秆纤维素、半纤维素、木质素含量，芒草细胞壁总成分73.42±2.18%，显著高于水稻54.03±2.23%，其中纤维素差异较小，半纤维素、木质素差异较大。NaOH预处理可以溶解半纤维素、木质素，暴露纤维素链，使得纤维素酶可以有效解除底物，提高酶解效率，酶解六碳糖产率随NaOH浓度提高逐渐增加，4.0%NaOH接近最高值，酶解液五碳糖的含量在1.0%NaOH达到最高值，总糖产率在4.0%NaOH达到最高值。芒草细胞壁组分含量高于水稻，导致在低浓度NaOH处理时总糖产率低于水稻，在高浓度木质素去除较彻底，总糖产率高于水稻。54256

毕业论文关键词：生物能源，芒草，水稻，NaOH预处理

Abstract：Biomass energy is a renewable and clean energy, is an ideal  alternative to fossil fuels. This experiment chooses Miscanthus sinensis (C4 plant) and rice (C3 plant) as material, through the pretreatment of NaOH, analysis the impact of changes in cell wall components of pretreatment and enzymatic hydrolysis sugar producing efficiency.

Straw cellulose, hemicellulose, lignin content was measured in the experiment, the total cell wall component of Miscanthus 73.42 ± 2.18%, significantly higher than that of rice 54.03 ± 2.23%, the small differences in cellulose，Semi cellulose, lignin differences. Pretreatment of NaOH could solubilize hemicelluloses, lignin, cellulose chain that exposure, enzyme can effectively relieve the substrate, improve the efficiency of enzymatic hydrolysis, enzymatic hydrolysis sugar yield of six with the increase of NaOH concentration gradually increased, 4.0%NaOH close to the highest , , content of five carbon sugars in enzyme solution reached the highest value at 1% NaOH,total sugar yield reached the highest value in the 4.0%NaOH. Miscanthus cell wall component content than rice, resulting in low concentration NaOH treatment total sugar yield was lower than that in rice, high concentration oflignin removed thoroughly, total sugar yield was higher than that of rice.

Keywords： Bioenergy, Miscanthus, Rice, NaOH pretreatment

目   录

1 绪论 1

1.1 生物质能 1

1.1.1 生物质概述 1

1.1.2 生物质能源的发展现状 2

1.2 植物细胞壁 2

1.3 细胞壁的化学组成 3

1.3.1 纤维素 3

1.3.2 半纤维素 4

1.3.3 木质素 4

1.3.4 果胶 5

1.4 细胞壁主要成分连接方式 6

1.5 生物质降解转化 7

1.5.1生物质降解转化流程 7

1.5.2 生物质预处理方式 7

1.5.3 生物质降解相关水解酶 8

1.6 本研究目的意义 9

2 材料与方法 9

2.1 实验材料 9

2.2实验方法 9

3 结果与分析 13

3.1 细胞壁成分测定 14

3.1.1 六碳糖、五碳糖标准曲线