水稻早期花药发育相关RING类E3连接酶的筛选
关键词：水稻；RING 类E3连接酶；花药发育；MPL；酵母双杂交
Screening for RING Type E3 Ligase Involved in Rice Early Anther Development
Abstract： RING (Really Interesting New Gene) type E3 ligase is a protein family containing zinc finger
domain that is capable of specifically recognizing a substrate and linking it to ubiquitin molecules. The
ubiquitinated substrate is then usually degraded in the 26S proteasome. It is known that
ubiquitin-proteasome pathway plays an important role in rice resistance to stresses, plant growth and
development. However, the role of ubiquitin-proteasome pathway in rice reproductive development
remains largely unclear. In this thesis, I screened E3 ligases involved in rice early anther development by
RNA-Seq data analysis and yeast two-hybrid. In our previous work，we identified a transcriptional
regulator MPL (MICROSPOROCYTELESS) plays an essential role in rice early anther development.
Disruption of MPL caused defects in formation of microspores and thus no pollen production. In order to
find E3 ligases that regulate MPL, I obtained 22 genes encoding proteins containing RING domain by
analyzing RNA-Seq data. I tested the interactions between MPL and 15 of the 22 E3 ligases by yeast
two-hybrid assays. No E3 ligases were found to interact with MPL. Then I used MPL as a bait to screen the
rice early anther cDNA library for E3 ligases involved in rice early anther development. I obtained eight
genes encoding proteins containing RING domain. The confirmation of interaction between these RING
protein with MPL is underway. These results provide valuable clues to identify novel RING-type E3 ligases
in early anther development and the molecular mechanisms of MPL regulation.
Key words: Rice; RING-type E3 ligase; Anther development; MPL; Yeast two-hybrid
摘要„1
关键词1
Abstract„1
Key words2
第一章 文献综述„2
1.1 水稻早期花药发育„2
1.1.1 水稻花药细胞分化过程2
1.1.2 水稻花药发育早期的调控„2
1.2 RING类E3泛素连接酶3
1.2.1 作用机理3
1.2.2 转录因子调控与自动反馈循环„3