DL-蛋氨酸对宫内发育迟缓哺乳仔猪肠道发育的影响
毕业论文关键词：DL-蛋氨酸；宫内发育迟缓；哺乳仔猪；肠道发育；抗氧化功能
Effects of DL-Methionine on intestinal development in intrauterine growth-retarded suckling piglets
Abstract: The present study was designed to evaluate the effects of DL-Methionine (DL-Met) on intestinal development in intrauterine growth-retarded (IUGR) suckling piglets. Five normal birth weight (NBW) and ten IUGR newborn piglets were selected and assigned to the following 3 experimental groups (n=5/group). Five normal birth weight piglets were fed a basic formula milk and the other 10 IUGR piglets were fed either a basic formula milk or a basic formula milk with + 0.12% DL-Met. The results showed that: Compared with NBW piglets, IUGR piglets had lower villus height and villus height/crypt depth ratio in ileum (P < 0.05). There were significant decreases in activities of lactase and sucrase in jejunum (P < 0.05). In addition, IUGR piglets had decreased glutathione (GSH) content and glutathione/glutathione ratio, whereas increased malondialdehyde (MDA) and protein carbonyl (PC) in jejunum (P < 0.05). Furthermore, the content of GSH was also significantly decreased in the ileum of IUGR piglets (P < 0.05). However, DL-Met treatment increased the villus height in both jejunum and ileum, and the villus height/crypt depth ratio in ileum (P < 0.05). DL-Met alleviated the decreased activity of sucrase in the jejunum of IUGR-MET piglets (P < 0.05). DL-Met supplementation significantly increased the GSH content, whereas decreased the PC content in jejunum (P < 0.05). These results indicated that DL-Met can alleviate the oxidative damage caused by IUGR and improve the intestinal development of IUGR suckling piglets.
Key words: DL-Methionine; Intrauterine growth-retardation; Suckling piglets; Intestinal development; Antioxidant function
目 录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 2
1 材料与方法 2
1.1 试验设计 2
1.2 样品采集 3
1.3 测定指标及方法 3
1.3.1 肠道组织形态变化的观察 3
1.3.2 肠道黏膜中二糖酶酶活性以及抗氧化功能的检测 3
1.3.3 肠道组织目的基因mRNA表达量的测定 3
1.4 数据统计与分析 4
2结果与分析 4
2.1 DL-蛋氨酸对IUGR哺乳仔猪肠道组织形态的影响 4