Y=48。91333+0。52417MT+0。093589AI+3。8×10-4MT×AI-3。85×10-3MT2-2。28×10-4AI2

2。 With the increase of MT concentration, the conversion rate of survival increased first and then decreased, and with the increase of AI concentration, it also increased first and then decreased, but the change rate was not significant in the set concentration range。 The linear effects of MT on survival was highly significant (P<0。01), the quadratic effects of MT and AI were significant (P<0。05), the linear effects of AI and the primary interactive effect of MT and AI were not significant on survival (P>0。05)。 The results of quadratic multiple regression analysis of experimental data and the actual quadratic regression equation of MT and AI concentration on survival of P。 ussuriensis were:

Y=88。3144+0。051055MT+0。06204AI+2。73×10-4MT×AI-2。01×10-3MT2-2。89×10-4AI2

3。 With the increase of MT concentration, the specific growth rate increased first and then decreased, and with the increase of AI concentration, it also increased first and then decreased。 The results showed that the linear and the quadratic effects of MT on SGR were highly significant (P<0。01), the linear and the quadratic effects of AI on SGR were significant (P<0。05), while the primary interactive effect of MT and AI were not significant on SGR (P>0。05)。 The results of quadratic multiple regression analysis of experimental data and the actual quadratic regression equation of MT and AI concentration on SGR of P。 ussuriensis were:

Y=1。87294+0。02837MT+4。68×10-3AI-3。17×10-6MT×AI-2。13×10-4MT2-1。22×10-5AI2

4。 Based on the 3 indexes are optimized at the same time, the optimal combination of the concentration of MT was 58mg。kg-1, the concentration of AI was 176mg。kg-1。 The specific growth rate was 3。21, the survival rate was 89。33%, the male rate was 85。18%。 The model reliability is 93。9%, which indicates that the model has high reliability and can provide theoretical guidance for the male production in P。 ussuriensis。

Keywords: Pseudobagrus ussuriensis, male rate, growth rate, survival rate, methyl testosterone, aromatase inhibitor

 目录

摘要 I

ABSTRACT III

一、 文献综述 1

1。1性转技术在鱼类中的应用 1

1。1。1 罗非鱼性别控制 1

1。1。2 黄颡鱼性别控制 2

1。1。3 其他鱼类 2

1。2乌苏里拟鲿性转技术研究现状 2

1。2。1性腺发育与性激素周年变化 2

1。2。2芳香化酶抑制剂对性腺及基因表达的影响 3

1。3本课题的目的及意义 3

二、 甲基睾丸酮与芳香化酶抑制剂对乌苏里拟鲿雄性率的影响 4

前言 4

2。1 材料及方法 4

2。2 结果 7

2。3 讨论 9

三、甲基睾丸酮与芳香化酶抑制剂对乌苏里拟鲿存活率的影响 11

前言 11

3。1 材料及方法 11

3。2 结果 13

3。3 讨论 15