不同来源大豆种质对细菌性斑点病抗性研究
毕业论文关键词：大豆；细菌性斑点病；抗源筛选；基因定位；抗病育种
A Study on Resistance of Soybean Germplasm to Bacterial Spot Disease from Different Sources
Abstract： Soybean bacterial spot is one of the main leaf diseases in soybean production in the world, and its pathogen is Pseudomonas syringae pv．glycinea. It is also the main disease in Northeast China. In this paper, 271 domestic and foreign soybean accessions were inoculated with soybean bacterial spot disease S1 strain. The resistance reaction level was investigated to reveal the variation of disease resistance and the whole genomic-wide association analysis was carried out using SNP molecular marker data obtained from SoyBase website. The results showed that there were differences in disease resistance in the tested materials. These accessions were classified into five types. Among them, no high resistance one was found and 47 and 8 lines with resistance and medium resistance levels were screened out respectively. Most of them are US early mature varieties. There were 216 lines with disease susceptibility level (including medium susceptible, susceptible and high susceptible), indicating the relatively low resistance of the test materials to S1 strain. SNP markers were utilized to map the QTL for the resistance. At the threshold of -log10P≥3, 21 SNP markers significantly correlated with resistance to S1 were detected and distributed on the 6 chromosomes. The marker Gm_15_146358 had the highest phenotypic explanation rate of 10.71%. The markers found in this study located in different regions in compared with the previous studies of resistance gene/QTL mapping. Our findings may lay the foundation for the research of genetic and breeding of soybean spot disease.
Key words: Soybean; bacterial leaf spot disease; resistance evaluation, gene mapping; resistance breeding
目 录
摘要 1
关键词 1
Abstract. 1
Key words 1
1 材料与方法 2
1．1 材料 2
1．2 方法 3
1．2．1 接种试验 3
1．2．2 抗病性鉴定与评价方法 3
1．2．3 关联分析方法 3
2 结果与分析 3
2．1 大豆种质接种细菌性斑点病S1菌株的症状反应 3
2．2 不同来源大豆种质对细菌性斑点病S1 菌株的抗性表现 4
2．3 大豆抗源的地理来源与熟期组 4
2．4 主成分分析 5
2．5 大豆对细菌性斑点病S1菌株抗性的关联分析 6
3 讨论 6
4 结论 7
致谢 7
参考文献 8
不同来源大豆种质对细菌性斑点病抗性研究