女贞子齐墩果酸和熊果酸提取工艺及抗氧化活性
毕业论文关键词：女贞子；齐墩果酸；熊果酸；微波提取；抗氧化
Study on the Extraction Technology of Oleanolic acid and Ursolic acid from Ligustrun Lucidum. and its Antioxidant Activities
Abstract: To explore the influence factors impacting extraction rate of oleanolic acid and ursolic acid from Ligustrun Lucidum, the optimal extraction conditions using microwave- assisted extraction were optimized by single factor and L9(34) orthogonal experiment, which included ethanol concentration, microwave power, the solid-liquid ratio, microwave-heating time and from the point of extraction rate of oleanolic acid and ursolic acid. At the same time, the oleanolic acid and ursolic acid content and antioxidant activity of fruits and stems-leaves was detected. The results showed that the optimal conditions were as follows: ethanol concentration 70%, microwave power was middle fire, the 1:25 ratio of solid-liquid, extracting for 3 min. The oleanolic acid and ursolic acid extraction rates of different parts of Ligustrun Lucidum were obtained under these conditions as follows: 0.674% of fruits, 0.873% of stem-leaf. It was also found that the extracts of Ligustrun Lucidum had different antioxidant activities. The clearance rates of superoxide anion radical in different parts of Ligustrun Lucidum were as follows: 91.4% of fruits, 84% of stem-leaf. The clearance rates of superoxide anion radical in different parts of Ligustrun Lucidum were as follows: 89.7% of fruits, 55.6% of stem-leaf.
Key words: Ligustrun Lucidum; Oleanolic acid; Ursolic acid; Microwave extraction; Antioxidant
目 录
摘要 1
关键词 1
引言 2
1 实验部分 2
1.1 实验所用材料、试剂及仪器 2
1.2 提取工艺流程 3
1.3 齐墩果酸的含量测定 3
1.3.1齐墩果酸标准溶液的配置 3
1.3.2 标准曲线的绘制 3
1.3.3 齐墩果酸提取率的测定 3
1.4 熊果酸的测定 4
1.4.1 熊果酸标准溶液的配置 4
1.4.2 标准曲线的绘制 4
1.4.3 熊果酸提取率的测定 4
1.5 单因素及正交试验 5
1.6 抗氧化活性 5
1.6.1 女贞子提取液对超氧阴离子清除活性 5
1.6.2 女贞子提取液对羟自由基清除活性 5
2 结果与讨论 5
2.1 单因素试验 5
2.1.1 乙醇浓度的选择 5
2.1.2 微波功率的选择 6
2.1.3 料液比的选择 7
2.1.4 微波时间的选择 7
2.2 正交试验结果 8
2.3 抗氧化试验结果 10
2.3.1大叶女贞叶、果实提取液对超氧阴离子自由基清除活性 10
2.3.2 大叶女贞叶、果实提取液对羟自由基清除活性 11
下一篇：落日黄色素对发酵后秸秆品质影响