聚乙二醇溶液浸种对栝楼种子萌发和幼苗生理特性的影响
毕业论文关键词：栝楼；聚乙二醇；种子萌发；生理特性
Effects of PEG Solution Soaking on Seed Germination of Trichosanthes Kirilowii Maxim and Seedling Physiological Characteristics
Abstract: Aiming at the problem of trichosanthes kirilowii seed germination difficult, under hydroponic conditions, to study the effects of polyethylene glycol (PEG)with different concentrations (0%, 10%, 15%, 20%, 25%, 30%) on trichosanthes kirilowii seed germination, seedling growth and related physiological activity. The results showed that, the low concentration of PEG (10%) treatment, trichosanthes kirilowii seed germination rate, germination potential, germination index, root length, plant height, fresh weight of seedling were higher than control, and reached a highly significant level, decreased when PEG concentration is higher than 10%,the trichosanthes kirilowii seed germination and seedling growth were inhibited under high concentrations of PEG (30%); At the same time, after the PEG solution treatment the seedlings of trichosanthes kirilowii, each physiological indexes in the seedling leaves were higher than control, including the MDA content increased, soluble protein, soluble sugar content and the enzyme activities of SOD and POD presented rising tendency then followed by decreasing . Under the light water stress, therefore, can effectively promote trichosanthes kirilowii seed germination and seedling growth, and the concentration 10% PEG was optimal.
Key Words:Trichosanthes Kirilowii Maxim; Polyethylene Glycol;Seed Germination；Physiological Characteristics
目 录
摘 要 1
Abstract 1
引言 2
1材料与方法 3
1.1实验材料 3
1.2处理方法 3
1.3 测定指标与方法 3
1.3.1幼苗生长指标 3
1.3.2幼苗生理指标 3
1.4 数据统计与分析 3
2结果与分析 4
2.1不同浓度PEG溶液浸种对栝楼种子萌发的影响 4
2.2不同浓度PEG溶液处理对栝楼幼苗生长的影响 4
2.2.1幼苗株高和根长的变化 4
2.2.2幼苗鲜重的变化 5
2.3 不同浓度PEG溶液处理对栝楼幼苗生理特性的影响 6
2.3.1幼苗叶片内MDA含量的变化 6
2.3.2幼苗叶片内SOD活性的变化 7
2.3.3幼苗叶片内POD活性的变化 8
2.3.4幼苗叶片内可溶性糖含量的变化 8
2.3.5幼苗叶片内可溶性蛋白含量的变化 9
3讨论 10
参考文献 11
致谢 14
聚乙二醇溶液浸种对栝楼种子萌发和幼苗生理特性的影响引言
栝楼（Trichosanthes kirilowii）俗名瓜蒌、吊瓜，为葫芦科栝楼属药用植物，其根（中药名天花粉）、果（中药名栝楼实）、果皮（中药名栝楼皮）、种子（中药名栝楼仁）均可入药[1]，具有很高的药用经济价值。据《中药大辞典》、《中国药物大全》、《本草图经》等中医药文献记载，栝楼甘、微苦、归肺经、可润燥滑肠、消肿排脓[2]。药理学进一步研究发现，天花粉中提取出的有效活性成分-天花粉蛋白、栝楼蛋白，是一类核糖体失活蛋白，能抗病毒、抗肿瘤[3]。栝楼籽富含脂肪、蛋白质，对高血压、高血脂、高胆固醇有辅助疗效，还能提高机体免疫功能，并有瘦身、美容等功效[4]。因此开发栝楼潜力大、经济效益高，除了具有药用价值外，还具有很高的食用价值。
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