利用转录组学研究甘薯响应黑斑病的分子机制
毕业论文关键词：甘薯黑斑病 抗性 分子标记 高通量测序 转录组测序
Molecular Mechanism in Response to the Black Rot Infection in Sweet Potato (Ipomoea batatas) by Using Transcriptome Analysis
Abstract Sweet potato is one of the most important crops and materials in industry. The black spot disease is one of the most serious threatens in sweet potato, which causes a huge lose to sweet potato’s quality and yield every year. At present, there are a lot of studies of field control for black spot disease, but few studies on its physiological characteristics and molecular mechanisms. The samples of strong and weak resistant varieties were sequenced by high-throughput sequencing to investigate sweet potato varieties’ resistance ability to black spot. This study is according to the depth and diameter of the infection disease to research their differential genes expression. We identifed 8 and 19 significantly differential expressed genes by high-throughput sequencing. This study uses the modern biological technology to find out the molecular basis of sweet potato black spot and have identified some candidate genes, which will provide the foundation for validating and further studying for these genes and then carrying out molecular breeding aiming to black spot.
Key words: Sweet potato black rot resistance, Molecular marker, High throughput sequencing, transcription sequencing
目录
摘要 . 1
Abstract . 1
目录 . 3
图清单 4
表清单 4
1 绪论 5
1.1 甘薯黑斑病病菌致病机理. 5
1.2 甘薯黑斑病的防治. 6
1.3 转录组测序概念及其过程. 6
2 材料与方法 .. 7
2.1 供试材料.. 7
2.1.1鉴定材料的繁殖 7
2.1.2菌液的制备 .. 7
2.2 接种方法.. 8
2.2.1接种工具的准备 8
2.2.2接种操作过程 . 8
2.3 转录组测序. 8
2.3.1RNA的提取 . 8
2.3.2cDNA 的制备与测序 .. 8
2.3.3数据处理 9
2.3.4从头组装 9
2.3.5基因注释 9
2.4 基因差异性分析.. 9
3 结果与分析 .. 10
3.1 极端数据结果与分析. 10
3.2 黑斑病相关抗性基因差异性分析.. 12
3.2.1DepthMean.r性状差异性分析结果 .. 12
3.2.2DiameterMean.r性状差异性分析结果 16
4 讨论 .. 20
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