水热处理对醇溶蛋白粘弹性的影响机制研究
毕业论文关键词：醇溶蛋白；热聚集；构象；粘弹性
Effect mechanism of hydrothermal treatment on the gliadin viscoelasticity
Abstract: The viscoelasticity of gliadin during heating could affect the initial expansion of dough during early baking process, thus determining the quality of the final product. In this paper, the aggregation behavior and conformational rearrangement of gliadin were studied under different hydrothermal temperature treatments to reveal the mechanism of gliadin viscoelasticity variations . The results showed that there was no covalent aggregation behavior of the gliadin during 40 °C and 60 °C hydrothermal treatment. At 90 °C, α-gliadin and γ-gliadin were aggregated by intermolecular disulfide bond (SS), resulting in a decrease in solubility in aqueous ethanol. During the hydrothermal treatment, the hydrogen bonds were gradually destroyed and the hydrophobic interactions were enhanced, resulting in the secondary structure changes. At 40 °C and 60 °C the rearrangement of non-covalent forces resulted in the gradual decrease in the viscosity of the gliadin. At 90 °C, the increase of protein elasticity was mainly due to the covalent aggregation behavior of gliadin, and the change of viscosity was due to the combined effect of noncovalent and covalent SS bond. This study could provide theoretical basis and scientific guidance for the regulation of glidin viscoelasticity in the initial stage of baking.
Keywords: Gliadin, thermal, polymerization, conformation, viscoelasticity
目 录
摘要 1
关键词 1
Abstract 1
Keywords 1
引言 1
1材料与方法 2
1.1试验材料 2
1.2 试验试剂 2
1.3仪器与设备 3
1.4试验方法 3
1.4.1醇溶蛋白的提取 3
1.4.2水热处理醇溶蛋白 3
1.4.3分子排阻高效液相色谱（SE-HPLC） 4
1.4.4游离巯基（SH）含量的测定 4
1.4.5反相高效液相色谱（RP-HPLC） 4
1.4.6醇溶蛋白在不同溶剂中的溶解度 4
1.4.7傅里叶变换红外光谱（FTIR） 4
1.4.8流变学研究 4
1.5统计分析方法 5
2 结果与分析 5
2.1热处理对醇溶蛋白分子量分布和游离SH的影响 5
2. 2热处理期间乙醇提取率和醇溶蛋白亚基分布变化 6
2. 3热处理对醇溶蛋白非共价力的影响 8
2. 4热处理对醇溶蛋白二级结构含量的影响 9
2. 5热处理对醇溶蛋白粘弹性的影响 10
3结 论 10