白桦脂酸的制备工艺研究
毕业论文关键词：白桦脂醇，氧化剂，白桦脂酸，产率
Abstract Abstract:Betulinic acid, a pentacyclic triterpenoids, can selectively kill melanoma cells and not kill healthy cells in the human body;Betulinic acid has inhibitory effect to infection of HIV-1, recent studies have showed that betulinic acid also has inhibitory effects to brain tumors, neuroectodermal tumors, leukemia and other cancer cells.This study is based on birch fatty alcohol as raw materials, potassium dichromate as the main antioxidant, will oxidize birch fatty alcohol to birch fatty acid synthesis. Wet oxidant betulin prepared by potassium dichromate, after full reaction, filtration, sedimentation, crystallization, can obtain relatively pure betulinic acid. In the preparation of betulinic acid synthesis, by controlling the synthesis of betulinic acid reaction conditions, such as birch fatty alcohol dosage, solvent dosage, reaction time, and so on,we can get the optimum reaction condition on synthesis of betulinic acid by observing which has the greatest effect among the reaction conditions on the yield of the synthesis. The research showed that the optimal reaction conditions: betulin 3.4mmol, oxidant 10mmol, molar ratio of 17:50, solvents 170mL, reaction time 2 hours,when at this reaction condition, the synthesis of betulinic acid, can be relatively at high yield, the highest yield was 79.7%.
Keywords:Betulin; Oxidant; Betulinic acid; Yield
目 录
1.国内外研究现状概述 1
1.1 白桦脂酸的来源： 1
1.1.1 .从植物中提取白桦脂酸 2
1.1.2.半合成方法制备白桦脂酸 3
1.1.3通过微生物发酵制备白桦脂酸 3
2.项目研究的目的及意义 4
3.材料与方法 5
3.1 实验材料与设备 5
3.2.合成方法 6
方案一合成白桦脂酸 6
方案二合成白桦脂酸 6
3.3 薄层层析检查 7
3.4 红外光谱仪测定 8
3.5 合成产率的单因素实验 9
（1）因素一 原料量——白桦脂醇对产率的影响 9
（2）因素二 溶剂——丙酮对产率的影响 9
（3）因素三 反应时间——搅拌时间对产率的影响 10
3.6 反应条件优化 10
4 结果与讨论 11
4.1 对两个实验方案的初步讨论 11
4.2 白桦脂醇用量对产率影响分析 13
4.3 溶剂丙酮对产率影响分析 15
4.4 反应时间对产率影响的分析 16
4.5最优化实验 17
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