不同种类包埋剂包埋花色苷的相互作用机理研究
毕业论文关键词：蓝莓；花色苷；蛋白；多糖；包埋
Interactions between different wall materials for the encapsulation of anthocyanins
Abstract: In this experiment, the protein (whey protein) and polysaccharide (β-cyclodextrin / Inulin) were mixed in different proportions (1: 1-4: 1) as wall materials for encapsulation of blueberry Anthocyanins, in order to study encapsulation of Anthocyanins and interactions between different wall materials. The result shows that, with the growing volume whey protein isolate, surface intention and electric conductivity of protein-polysaccharide solution are going lower and always lower than the calculated value. So there is interaction between the protein and polysaccharide in the barrier agent, which is probably due to the presence of C-O in the composite solution. Between the wall material and core material, there also exist the interaction force, which possibly due to the existence of N-H. When the whey protein and inulin were mixed with blueberry anthocyanin in the ratio of 4: 1 (w / w) as barrier agent, the highest encapsulation production and encapsulation efficiency were obtained. The result of this research will have a profound influence on practical productivity of Anthocyanins microencapsulation.
Key words: Blueberry; Anthocyanins; protein; polysaccharide; encapsulation
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法4
1.1材料与试剂 4
1.2仪器与设备 4
1.3试验方法4
1.3.1蓝莓花色苷提取物制备 5
1.3.2蛋白-多糖复合溶液配制5
1.3.3花色苷微胶囊的制备 6
1.3.4检测指标及方法 6
1.4数据处理及分析6
2 结果与分析7
2.1包埋剂表面张力分析8
2.2包埋剂电导率分析9
2.3包埋中单一物质的红外光谱10
2.4花色苷微胶囊表面张力分析11
2.5花色苷微胶囊电导率分析11
2.6花色苷微胶囊红外光谱 12
2.7花色苷微胶囊包埋产率12
2.8花色苷微胶囊包埋效率13
3 结论 13
致谢13
参考文献14
不同种类包埋剂包埋花色苷的相互作用机理
花色苷(anthocyanins)是自然界最重要的一种水溶性的色素之一，分布于 27 个科，72 个属的植物中[1]。蓝莓中含有丰富的花色苷，它不仅利于授粉和种子传播、抵抗植物虫害[2]以及预防植物的紫外线照射损伤[1],而且对于人类具有许多生理保健功能,如清除体内自由基[3-4]、抗肿瘤、抗癌[5-10]、抗炎、抑制脂质过氧化和血小板凝集、保护肝脏、预防糖尿病、减肥、保护视力等[11]。
包埋是指用其他物质（包埋剂）包裹活性物质（芯材），并将其粒径从纳米级转化为毫米级。包埋过程中，壁材于芯材经过均匀混合再进一步冷冻干燥或喷雾干燥。可用来进行包埋的材料有碳水化合物，亲水胶体，蛋白质，纤文。例如，Flores[12]的研究中用WPI作包埋剂。Turan[13]的研究中用阿拉伯胶和环糊精的混合物进行实验包埋蓝莓中的生物活性物质。基于这些试验结果，包埋壁材的组成对包埋效率，包埋产率，花色苷中的功能性物质有深远影响。