摘要傅里叶合成孔径显微技术（Fourier ptychographic microscopy, FPM）是最新研究的一 种大视场、高分辨显微成像技术。与传统的合成孔径显微技术不同，FPM 是在傅里叶频域 中将一系列低分辨率图像进行熔合，得到一张正确的高分辨率、高空间带宽积的输出图像。 该技术可以大大提高光学系统的空间带宽积，实现大视场和高分辨率的兼顾，并且不需要 机械扫描和相位测量。本毕业设计拟开展基于可编程照明的大视场高分辨计算显微成像技 术的研究：原理上基于迭代相位恢复算法的定量相位显微成像理论；硬件上依托以 LED 点阵为基础的光学平台；软件上使用 MATLAB 程序语言，进行算法处理以及基本人机交 互界面的实现。该课题最终在低倍物镜下结合现有显微镜系统，实现生物细胞的大视场、 高分辨的吉比特像素成像。77965

毕业论文关键词 FPM 大视场 高分辨 MATLAB

毕业设计说明书外文摘要

Title Research  on  wide-field,high-resolution computational microscopic imaging which based on programmable lighting。

Abstract

Fourier ptychographic microscopy(FPM) is a newly developed method which can achive both wide-field and high-resolution。Unlike traditional ptychography method,it works by iteratively stitching together a number of low-resolution images in Fourier space to recover an accurate high-resolution,high-SBP output image。FPM can not only greatly improve the SBP of optical system, achive both wide-field and high-resolution, but also remove the need for mechanical scanning and phase measurement。This project plans to research on this wide-field and high-resolution computational microscopic imaging method which based on programmable lighting。The principle of this project is quantitative phase microscopy imaging theory of iterative phase recovery algorithm。The hardware of this project is relying on an optical platform which based on an LED array。The software which is programmed by MATLAB can implement basic algorithms and human-computer interaction interface。 Eventually,combined with the existing microscopic system,we can achive wide-field,high-resolution and gigapixel imaging of biological cells with a low-power objective lens。

Keywords    FPM  wide-field  high-resolution MATLAB

本科毕业设计说明书 第 I 页

目 次

1  绪论 1

1。1  研究背景 1

1。2  国内外研究现状 2

1。3  研究目的及意义 3

1。4  研究内容 4

2 FPM 显微成像原理及系统简介 5

2。1 FPM 显微成像原理 5

2。1。1 基于迭代相位恢复算法的定量相位显微成像理论 5

2。1。2 FPM 显微成像基本原理简介 6

2。1 FPM 硬件系统介绍 8

2。2。1 FPM 显微成像系统的硬件电路 8

2。2。2 可编程 LED 阵列照明系统 9

2。2。3 1951 USAF 分辨率测试板 12

3 FPM 显微成像的具体实现