共生菌沃尔巴克氏体对杂拟谷盗生殖的影响
毕业论文关键词：杂拟谷盗；沃尔巴克氏体；繁殖；胞质不亲和性
Effect of symbiotic bacteria Wolbachia on the reproduction in Tribolium confusum
Abstract: Wolbachia is a kind of widespread symbiotic bacteria. To clarify the influence of Wolbachia on host reproduction in the Tribolium confusum , and to konw if it was a symbiotic relationship between the symbiotic bacteria and the host tribolium confusum, we first eliminated it from beetles by feeding them on tetracycline flour; then we determined its influence on host reproduction by the treatment of four mating combinations. The results showed that Wolbachia existed in tribolium confusum’s body, and they could be suppressed and removed by feeding tetracycline flour. Wolbachia did not play roles in host eggs production and female ratio, whereas they played roles in hatching rate, pupation rate. Wolbachia could cause host cytoplasmic incompatibility between sperm and egg, and there was a symbiotic relationship between Wolbachia and host T. confusum. This study has both the important theory significance and the application value in practice, which provides a base for further research in T. confusum.
Key words: Tribolium confusu；Wolbachia; reproduction; cytoplasmic incompatibility
目录
引言 1
1 材料与方法 2
1.1 供试昆虫 2
1. 2杂拟谷盗体内沃尔巴克氏体的抑制消除试验 2
1.3 沃尔巴克氏体对杂拟谷盗繁殖的影响及其互作关系 3
1.4 数据统计与分析 3
2结果与分析 3
2.1 杂拟谷盗体内沃尔巴克氏体的抑制性消除结果 3
2.2 沃尔巴克氏体对杂拟谷盗繁殖的影响及其互作关系 4
3.讨论 4
致谢： 8
参考文献 8
引言
Wolbachia又称为沃尔巴克氏体，属于变形菌纲，立克次氏体科、乌巴克体族、乌巴克体属中的一种，上个世纪二十年代由研究人员在蚊子的生殖组织里首次被发现[1]。这些细菌通过卵的细胞质垂直转移，并且参与其寄主的生长发育、交配与生殖等多种生理功能及其调控，这种调控对Wolbachia自身的水平和垂直转移以及寄主的生长发育、交配和繁衍具有重要作用。
随着对Wolbachia研究的深入，越来越多的结果表明Wolbachia在自然界中有着非常广泛的分布[2-4]。Wolbachia能够感染线虫[5-7]以及昆虫、甲壳动物、螨[8]等多种节肢动物宿主，不仅如此，Wolbachia在包括鞘翅目、双翅目、半翅目、鳞翅目、弹尾目等昆虫体内都有Wolbachia的存在。据研究统计，受感染的昆虫种类大约占70%，由此可见，Wolbachia是一种在世界范围内中昆虫体内分布范围非常广，存在丰富度也非常大的内共生菌[9]。可以通过抗生素来去除寄主体内的Wolbachia[10-11]，也可通过高温等物理方法进行Wolbachia的去除[12]。目前，大多都用这些方法来获得体内没有Wolbachia的昆虫。