摘要用组织分离法和纯培养方法从江苏师范大学泉山校区杜仲中分离内生菌，并对其进行保种以及菌株鉴定，了解药用植物杜仲内生菌的生物多样性。共分离纯化出内生菌89株，包括16株放线菌、39株真菌和34株细菌。并对34株细菌和16株放线菌的功能进行了初步研究，结果表明34株内生细菌均有抗甘薯长喙壳菌、茄腐镰刀菌的活性，16株放线菌中的2株放线菌（Eua-01-G3、Eua-02-J8）表现了较强的抑菌活性，经鉴定2株都属于链霉菌属。酶活实验测定表明34株内生细菌均有产酶活性，其中13株有4种产酶活性，11株有3种产酶活性，6株有2种产酶活性，而4株内生细菌仅有1种产酶活性。该研究为内生菌的应用提供了一定的理论依据。50710

该论文有图8幅，表23个，参考文献23篇。

毕业论文关键词：杜仲  内生菌  多样性  抑菌活性  产酶活性

Diversity of Endophytes Associated Eucommia Ulmoides and Their Bioactivities Against Pathogenic Fungi

Abstract

Using tissue separation and pure culture method to separate the endophytes associated Eucommia ulmoides from Jiangsu Normal University Quanshan campus and carrying on the storage and strain identification, we manage to study the biopersity of endophytes associated Eucommia ulmoides. In my study, 89 strains endophytes were successfully separated and purified, including 16 strains endophytic actinomycetes, 39 strains endophytic fungi and 34 strains endophytic bacteria. Then, the function of the above 34 strains bacteria and 16 strains actinomycetes was researched preliminarily. The results showed that 34 strains endophytic bacteria have resistance to Ceratocystis fimbriata and Fusarium solani, and two of the sixteen strains actinomycetes (Eua-01-G3, Eua-02-J8) showed strong antifungal activity, belonging to Streptomyces. Enzyme activity experiment showed that 34 strains endophytic bacteria all can produce active enzyme, of which 13 strains had 4 kinds of enzyme-producing activity, 11 strains had 3 kinds of enzyme-producing activity, 6 strains had 2 kinds of enzyme-producing activity, and the remaining 4 strains have only one kind of enzyme-producing activity. The research for the application of endophytes have provided the certain theory basis.

Key Words: Eucommia ulmoides  endophytes  persity  antifungi activity     enzyme-producing activity

 目  录

摘要 III

Abstract IV

目录 V

图清单 VI

表清单 VI

1 引言 1

2 材料与方法 3

2.1试验材料与设备 3

2.2方法 7

3 结果与分析 10

3.1 杜仲内生菌的分离纯化 10

3.2 抑菌活性 12

3.3  34株细菌的水解酶活性 12

4 讨论 17

参考文献 18

致谢 20

图清单

图序号 图名称 页码

图3-1 真菌形态图

细菌形态图

图3-2 细菌形态图

放线菌形态图 11

图3-3 放线菌形态图