次甲基蓝作为电子穿梭载体提高偶氮染料脱色效率的研究
毕业论文关键词：Shewanella oneidensis MR-1 次甲基蓝 电子穿梭载体 偶氮脱色
The Study of Methylene blue Acting as Electronshuttles in Accelerating the Decolorization of Azo dye
Abstract [Objective] High-efficiency, low-cost and non-toxicity electron shuttles wereselected to accelerate the anaerobic decolorization of Congo red by Shewanellaoneidensis MR-1. [Methods] Under the anaerobic condition, Shewanella oneidensisMR-1was employed to decolorize 50 mg/L Congo red at 35 ºC and pH 6.5. Controlswith inactivated cells were performed under the same conditions. To evaluate thecapabilities of electron shuttles in promoting anaerobic decolorization of Congo red, 1,2, 4 and 8 μM acid yellow, amino black, methyl violet, naphthol green, crystal violetand methylene blue were added into the anaerobic decolorization system respectively.[Results] S. oneidensis MR-1 could decolorize 50 mg/L for Congo red completelywithin 8 h. However, the inactivated cells failed in decolorizing Congo red. Thedifferent concentration of amino black, methyl violet, crystal violet, acid yellow andnaphthol green could not accelerate the decolorization of Congo red. 1, 2, 4 and 8 μMmethylene blue increased 14.6%, 19.2%, 31% and 46.6% decolorization efficienciesrespectively. [Conclusion] The results indicated that biodegradation, rather thanbioasorption, induced by MR-1 cells mainly contributed to the decolorization ofCongo red. In addition, methylene blue could accelerate the decolorization of Congored in a concentration dependent manner.
Key words: Shewanella oneidensis MR-1 methylene blue electron shuttlesazo dye decolorization
目录
摘要 I
Abstract- II
目录 III
1 引言-1
1.1偶氮染料的概念与危害 1
1.2偶氮类染料废水常用的处理方法 1
1.3希瓦氏菌对偶氮染料的降解- 3
1.4电子穿梭载体强化偶氮染料的降解-3
1.5研究目的与内容-4
2 材料与方法 4
2.1实验材料- 4
2.2实验方法- 8
3 结果与分析 8
3.1 S. oneidensis MR-1 在 pH6.5 以及灭活条件下脱色刚果红效果 8
3.2高效电子载体的筛选结果-9
4 讨论 12
参考文献-13
致谢15
1引言1.1偶氮染料的概念与危害我国是纺织印染业的第一大国， 印染废水的排放量约占整个工业废水的 35%且回收率不到10%[1]。偶氮染料是工业染料中使用量最大的一类，其产量占染料总量 70%左右[2]。在偶氮染料的结构中始终存在一个或多个与萘环或苯环相连的偶氮键 (-N=N-) ，在这些萘环或苯环上面同时连有-NH2、-OH、-SO3、-CH3和-NO2等一些极性基团，这种结构特征造成了偶氮染料结构的多样性[3]。偶氮染料化学性质稳定，在自然环境中难以彻底降解，若不经处理直接排放到水域中会破坏水生环境，极大的威胁了人类和其他生物的健康 [4]。1.2偶氮类染料废水常用的处理方法近年来，研究最多的偶氮染料废水处理的方法有：物理法、生物法、化学法等[5]，随着污染的加剧，单一的处理方法难以有效地脱色印染废水，因此将不同的处理方法相偶联，如：化学-生物法、物理-生物法、物理-化学法等也成为今后的研究热点[6]。
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