摘要：近年来，乳腺癌发病率和死亡率呈迅速增长的趋势，因此探究肿瘤转移已成为重要研究课题。现今基因治疗的崛起为癌症治疗带来了更多的机会。CERS2基因为肿瘤抑制基因，它与癌细胞的凋亡与转移密切相关。本文目的：探究干扰CERS2表达对乳腺癌MCF7细胞转移有何影响，为乳腺癌的临床治疗提供新思路。方法及内容：实时定量PCR实验确定乳腺癌细胞MCF7；通过穿孔实验观察干扰CERS2表达对乳腺癌细胞迁移和侵袭的影响。结果表明：干扰CERS2表达会促进乳腺癌细胞的迁移和侵袭。51841

该论文有图6幅，参考文献11篇。

毕业论文关键词：乳腺癌、CERS2、抑制、转移

Effect on the breast cancer cell migration and invasion by interfering CERS2 expression

Abstract： In recent years, the breast cancer incidence and mortality showed a rapid growth trend, so exploring metastasis mechanism of cancer cells has become an important research topic. Today, the rise of gene therapy for cancer treatment has brought us more opportunities. CERS2 is a tumor suppressor gene, which is closely related to cancer cell apoptosis and metastasis. Objective: To explore the effect on the migration and invasion of MCF7 cell by interfering CERS2 expression; To provide the new idea for the clinical treatment of breast cancer. Method and Content: In the first, we determined the breast cancer cell line MCF7 by real-time PCR. Next, we observed the effect on the breast cancer cell migration and invasion by transwell experiment. The results showed that the migration and invasion abilities of breast cancer cell were promoted by interfering CERS2 expression.

Key words: breast cancer; CERS2; migration; invasion

目 录

摘要 I

Abstract II

目 录 III

图清单 III

前言 IV

1材料与方法 1

1.1细胞株与细胞系 1

1.2实时定量PCR（Real-time PCR） 1

1.3 蛋白提取和免疫印迹（Western Blotting） 1

1.4 Transwell实验 2

2 结果与分析 3

2.1 乳腺癌细胞株中CERS2表达情况 3

2.2 干扰片段的选择 3

2.3 干扰CERS2对乳腺癌细胞迁移和侵袭的影响 4

2.4 干扰CERS2对细胞外氢离子浓度的影响 5

3 讨论 6

致谢 7

参考文献 7

图清单

图序号 图名称 页码

图1 乳腺癌细胞株中CERS2的表达情况 3

图2 干扰CERS2后，MCF7的核酸水平及蛋白水平 4

图3-1 干扰CERS2对癌细胞迁移的影响 4

图3-2 干扰CERS2对癌细胞侵袭的影响 5

图4-1 细胞外pH标准曲线 5

图4-2 干扰CERS2表达后细胞外的氢离子浓度与对照组细胞相比较