土霉素的水生生物毒性研究+文献综述
Study on toxicity of Oxytetracycline in Microcystis aeruginosa
Abstract: The immediate impact of Oxytetracycline (OTC), one kind of tetracycline antibiotics, on microcystin-LR (MC-LR) release of Microcystis aeruginosa was investigated. The relationship between absorbance and cell density, determined by measuring cell density and using UV spectrum, is for counting cell numbers per L. MC-LR concentration released by Microcystis aeruginosa was detected by Indirect Enzyme -Linked ImmunoSorbent Assay (Indirect ELISA) after exposure to a various concentration range of OTC. In a 48 h cultivation after treated by 1 ppm, 2 ppm and 5 ppm OTC solution, the cell density increased but the increment was lower than control sample, and the cell density decreased as concentration of OTC solution increased. In the cultivation after treated by 10 ppm OTC solution, the cell density decreased. In the cultivation after treated by 5 ppm OTC solution, the extracellular MC-LR concentration, total MC-LR concentration and the release of MC-LR per 105 cells were all lower than other samples. In the cultivation after treated by 1 ppm OTC solution, the extracellular MC-LR concentration, total MC-LR concentration and the release of MC-LR per 105 cells were all higher than other samples. The total release of MC-LR and the intracellular MC-LR concentration increased as concentration of OTC solution increased. Study found that residual OTC in the environment has an immediate impact on MC-LR release of Microcystis aeruginosa. So we should make rational use of antibiotics.
KeyWords: Oxytetracycline; microcystin-LR (MC-LR); tetracycline antibiotics; ELISA
目录
1. 引言 1
1.1 抗生素简介 1
1.1.1 四环素类抗生素结构 1
1.1.2 抗生素污染现状 1
1.1.3 土霉素的环境行为 2
1.1.4 土霉素污染现状 2
1.2 微囊藻毒素研究现状 3
1.2.1 MC-LR的结构性质 3
1.2.2 微囊藻毒素理化性质 4
1.2.3 微囊藻毒素与水生生物间的互相影响 4
1.2.4 MC-LR毒理作用 5
1.3 本研究内容、目的和意义 6
2. 材料和方法 6
2.1 仪器灭菌 6
2.2 BG-11培养基配制 7
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