摘要本报告描述了被表面活性剂包覆的石墨化多壁碳纳米管（SC-GMWNTs）的使用，被表面活性剂包覆的石墨化多壁碳纳米管（SC-GMWNTs）在带有二极管阵列的毛细管电泳中作为新型伪静止相，用于检测酚酸和丹参酮草药和尿液样品。同时，还对影响分离的几个参数进行了研究，如十二烷基硫酸钠的浓度（SDS）；石墨化多壁碳纳米管（GMWNTs）；异丙醇；碳纳米管；硼酸钠含量和缓冲液的选择等。结果显示，由于石墨化多壁碳纳米管（GMWNTs）的表面和靶化合物之间存在强相互作用，被表面活性剂包覆的石墨化多壁碳纳米管（SC-GMWNTs）在缓冲液运行中相对于传统的微胶粒的靶分析物来说，有效提高了分离效率。在适合的条件下，这种方法表现出良好的线性关系，相关系数大于0.9950，检出限的范围为0.71-3.10微克/毫升。此外，当10mM硼酸，30mM的十二烷基硫酸钠的浓度（SDS），10％的异丙醇和6微克/毫升的石墨化多壁碳纳米管（SC-GMWNTs）被引入缓冲溶液时，达到了令人满意的分离效果，分离恢复值在89.97％-103.30％的范围内。47836

Abstract

This report describes the use of surfactant-coated graphitized multi-walled carbon nanotubes (SC-GMWNTs) as a novel pseudostationary phase in capillary electrophoresis with diode array detection for the determination of phenolic acids and tanshinones in herbal and urine samples. Several parameters influencing the separation were studied,such as the concentrations of sodium dodecyl sulfate (SDS); GMWNTs, and isopropanol; choice of carbon nanotubes; sodium borate content and buffer pH.The results revealed that the presence of SC-GMWNTs in buffer enhanced the separation efficiency for the target analytes relative to conventional micelles due to the strong interaction between the surface of the GMWNTs and the target compounds. Under the optimum conditions, the method showed good linearity, with correlation coefficients higher than 0.9950. Limits of detection were in the range of 0.71-3.10 μg/mL. Furthermore, satisfactory separations were achieved with good recovery values in the range of 89.97% and 103.30% when 10 mM borate, 30 mM SDS, 10% isopropanol, and 6 μg/mL SC-GMWNTs were introduced into the buffer solution.

毕业论文关键词：胶束电动色谱； 伪静止相； 丹参； 被表面活性剂包覆的石墨化多壁碳纳米管；尿样

Keywords: Micellar electrokinetic chromatography, Pseudostationary phase, Salvia miltiorrhiza, Surfactant-coated graphitized multi-walled carbon nanotubes, Urine

目录

1. 引言 5

2. 实验部分 6

2.1实验试剂 6

2.2实验仪器 7

2.3实验过程与讨论 7

2.3.1材料表征 7

2.3.2毛细管电泳条件 8

2.3.3 缓冲液制备过程 8

2.3.4对照品溶液制备过程 9

2.3.5药材样品制备过程 9

2.3.6 分散液浓度考察 10

2.3.7分散液种类考察 11

2.3.8 十二烷基硫酸钠的浓度（SDS）浓度考察 12

2.3.9异丙醇浓度考察 14

2.3.10硼砂浓度考察 15

2.3.11 pH考察 16

2.3.12 电压考察 17

2.3.13 温度考察