多裂鱼黄草组织培养初探+文献综述
毕业论文关键词： 旋花科；观赏植物；培养基的选择；激素配比；愈伤组织形成
The tissue culture of Merremia dissecta
Abstract: The Convolvulaceae has populations in tropics、semitropics and temperate zone of eastern and western hemispheres in earth,which produces chiefly in tropics and semitropics of the American Continent and Asia.There are more than 22 genus and 130 species in China,which is in both south areas and north areas.But majority of the convolvulaceae produces in southwest and southern part of China.Many of them are vegetables and cash crops.Lots of them are medicinal and ornamental plants.But the convolvulaceae lacks the systematic study of tissue culture about Merremia dissecta. Through choosing the culture mediu, mixing the hormone,we discuss the technology of tissue culture about Merremia dissecta.Finally,we choose the best training program.The concentration of 6-BA of 3mg/L and the concentration ofNAAof 0.1mg/L is the best training program to the tissue culture of Merremia dissecta,on matter whether the stem tip、the stem or the blade of the Merremia dissecta.
Key Words: convolvulaceae;ornamental plant;the choice of medium;hormone combination;callus formation
目录
1 绪论 1
1.1 旋花科植物概述 1
1.1.1 旋花科植物的分类和分布 1
1.1.2 旋花科植物的形态特征 1
1.2 多裂鱼黄草概述 2
1.2.1 多裂鱼黄草的形态特征 2
1.2.2多裂鱼黄草的生态性和应用 2
1.2.3多裂鱼黄草植物国内外研究现状 2
1.3 植物组织培养技术 2
1.3.1 植物组织培养的定义和目的 2
1.3.2 植物组织培养发展简史 2
1.3.3 植物组织培养技术的前景展望 4
1.4 多裂鱼黄草的研究意义 5
2 材料与方法 5
2.1 实验材料 5
2.2 实验试剂 5
2.3 实验仪器设备 5
2.4 实验常用器皿、器械 6
2.5 实验方法 6
2.5.1 配置激素 6
2.5.2 MS培养基配制、灭菌与保存 6
2.5.3多裂鱼黄草的初代培养 7
2.5.4 多裂鱼黄草的继代培养 9
3 结果与分析 10
3.1 外植体的变化 10
3.2 不同浓度6-BA和NAA组合对多裂鱼黄草茎尖愈伤组织诱导的影响 14
3.3 不同浓度6-BA和NAA组合对多裂鱼黄草茎段愈伤组织诱导的影响 14
3.4 不同浓度6-BA和NAA组合对多裂鱼黄草叶片愈伤组织诱导的影响 15