摘    要：杭白菊是原产于浙江桐乡的一种重要药食两用植物, 具有很高的药用价值和保健功效，其主要有效活性成分是多酚类化合物等，目前已经成为药品、食品等工业产品的重要原料。本研究以我省道地产区杭白菊为实验材料,利用LC/MS对杭白菊中生物活性成分进行全谱测定，首次对道地杭白菊花和叶片中的生物活性成分进行全面分析。结果表明，采用正、负离子（ESI+、ESI-）检测模式分别从杭白菊样品中分离鉴定出64种和56种多酚类物质；叶片和花中含有类似多酚类物质，但花中含有的多酚类物质种类较叶中多，而叶片中咖啡酸、芹黄素、橘皮苷、毛地黄等黄酮类物质的相对含量相比花中高。上述实验结果对道地产区杭白菊花及其制品的质量控制，以及对杭白菊叶片的综合开发利用，提高产业的综合效益具有一定的指导意义。

Hangzhou white chrysanthemum is a kind of important originating in tongxiang city, zhejiang province, medicine and food plants has high medicinal value and health care effect, the main effective active ingredient is polyphenol compounds and so on, at present has become the important raw materials in pharmaceutical, food and other industrial products. This study authentic production areas in our province and hangzhou white chrysanthemum as experimental material, the use of LC/MS of bioactive ingredients in hangzhou white chrysanthemum full spectrum measurement, for the first time of authentic hang white chrysanthemum and biological active ingredient to conduct a comprehensive analysis of blade. The results showed that 64 species and 56 polyphenols were identified by using positive, negative ion (ESI+, ESI-) detection patterns. Leaves and flowers contain polyphenols, but contain more kinds of polyphenols than in the leaf, and caffeic acid in the leaves, apigenin, hesperidin, foxglove, the relative content of flavonoids of high compared to the flowers. The above experimental results have certain guiding significance for the quality control of the chrysanthemum and its products in the daodi region, as well as the comprehensive development and utilization of the leaves of the chrysanthemum.

关键词：杭白菊；生物活性成分；成分测定；LC—MS ；正、负离子检测模式

Keyword: Hangzhou white chrysanthemum; Bioactive components; Component determination; LC – MS；Positive and negative ion detection mode.

目录

1．引言 5

1.1 杭白菊简介 5

1.2 杭白菊中的活性成分 5

1.2.1 黄酮类 5

1.2.2 挥发油类 5

1.2.3 多糖类、氨基酸类、微量元素以及其他成分 5

1.3 杭白菊的功效 5

1.4 杭白菊在食品工业上的应用 5

1.5 杭白菊提取工艺优化 6

1.6 杭白菊研究现状 6

2.实验操作与过程 6

2.1材料来源 6

2.3杭白菊样品预处理 6

2.4 LC/MS分析 6

3.结果分析及讨论 7

3.1 数据分析 8

3.2 全谱鉴定结论 13

3.3 分析及讨论 13

4.展望 14

参考文献