摘要:  本论文以里氏木霉Rut C-30（Trichoderma reesei）为产酶菌种，研究了不同氮源对里氏木霉产纤维素酶的影响。分别在固定硫酸铵含量1.4g/L或5g/L 的基本培养基分调节玉米浆（CSL）的浓度（5、3、2、1g/L）来测定纤维素酶酶活随时间的变化；在含有不同浓度的硫酸铵的基本培养基测定酶活随时间的变化（5、3、2、1.4g/L）；在含有不同浓度的尿素（0.1%、0.2%、0.3%、0.4%、0.5%）的基本培养基测定酶活随时间的变化。实验表明不同的氮源对里氏木霉产纤维素酶影响不大，其中硫酸铵含量1.4g/L 的基本培养基CSL浓度为1g/L的酶活最高为6.07U/ml；而硫酸铵含量为5g/L的培养基中CSL浓度为2g/L的酶活最高为6.02U/ml；当硫酸铵浓度为3g/L时酶活最高为5.87U/ml；0.3%的尿素能够有效的提高产酶能力。5吹冰6

毕业论文关键词：里氏木霉，氮源，纤维素酶，酶活

Abstract: This thesis uses Trichoderma reesei Rut C-30 as cellulase production strain to study the effects of different nitrogen sources on the production of cellulase. Basic medium with 1.4g/L or 5g/L ammonium sulphate of corn steep liquor (CSL) concentration (5, 3, 2, 1g /L) to determine the change of enzyme activity over time; Enzyme activity was determined over time in basic medium containing different concentrations of ammonium sulfate (5, 3, 2, 1.4g /L) or different concentrations of urea (0.1%, 0.2%, 0.3% , 0.4%, 0.5%). Experimental results show that the different nitrogen sources do not have much effect on the T. reesei cellulase production. Basic medium with 1.4g/L ammonium sulfate has the highest activity when CSL concentration is 1g/L; While Basic medium with 5g/L ammonium sulfate has the highest activity when CSL concentration is 2g/L; When changing ammonium sulfate concentration, 3g/L ammonium sulfate gives maximum enzyme activity; When changing urea concentration, 0.3% urea gives the highest cellulase activity.

Keywords: Trichoderma reesei, nitrogen source, cellulase, enzyme activity

目录

1 前言 2

1.1研究背景 2

1.2立题依据 2

2材料与方法 3

2.1菌种与药品 3

2.2培养基 3

2.2.1 PDA培养基 3

2.2.2基本培养基 5

2.3试剂 6

2.3.1DNS试剂 6

2.3.2柠檬酸缓冲液 7

2.4实验仪器 7

2.5菌种制备 7

2.6产酶培养基优化 7

2.7绘制葡萄糖标准曲线 8

2.8酶活测定 8

2.9酶活计算方法 9

3结果与分析 9

3.1葡萄糖标准曲线 9

3.2硫酸铵1.4g/L的培养基中不同CSL浓度对产纤维素酶的影响 10

3.3硫酸铵5g/L的培养基中不同CSL浓度对产纤维素酶的影响 11

3.4不同硫酸铵浓度对产纤维素酶的影响 13

3.5不同浓度尿素对里氏木霉产纤维素酶的影响 14

3.6分析 16

结论 18

参考文献 19