猪源大肠杆菌对常用抗菌药的耐药性分析
毕业论文关键词：猪源大肠杆菌；耐药性；抗菌药；MIC；PCR
Analysis of drug resistance of Escherichia coli to commonly used antibiotics in veterinary clinics
Abstract： Escherichia coli is not only an important pathogen of humans and animals, but also an important symbiotic bacteria. As a drug-resistant gene storage, it is often regarded as a drug-resistant indicator bacteria. Drug-resistant Escherichia coli from animal-derived may be transmitted to humans through a variety of routes, and drug-resistant genes may be transferred in different strains, leading to treatment failure and threatening human health. It is necessary to monitor the resistance of Escherichia coli. In this paper, we investigated the drug resistance of Escherichia coli isolated from different pig farms in Jiangsu. A total of 83 swine samples were collected and finally 43 strains of Escherichia coli were isolated and identified. Minimum inhibitory concentrations (MICs) of ten commonly used antimicrobial agents against E. coli strains were detected The results showed that E. coli strains isolated from pigs in Jiangsu province presented serious resistance to common antibiotics, especially to sulfadiazine, ampicillin and florfenicol, and the resistant rate was up to 100%. Other 8 commonly used antibiotics also showed resistance to some degree. Further analysis show that it existd multiple drug resistance phenomenon and multi-drug resistance profiles were complex and perse. The high frequency of multi-drug resistant profiles was mainly to 9 kinds of antimicrobial resistance. The results suggest that the resistance of E. coli isolated from different pig farms is becoming more and more serious. It should not only pay attention to the pig farms, but also provide the basis for establishing the database of bacterial resistance in pig farms.
Key words: Escherichia coli; drug resistance; antimicrobial agents; MIC; PCR
目 录
摘要.1
关键词.1
Abstract..........1
Key words..1
引言（或绪论）.1
1 材料与方法.................2
1.1材料...2
1.1.1主要器材和设备...2
1.1.2抗菌药及其配置过程...2
1.1.3培养基及其制备...3
1.2方法...2
1.2.1细菌的分离和鉴定...3
1.2.1.1样品采集 ..........3
1.2.1.2细菌培养....................3
1.2.1.3初步鉴定....................3
1.2.1.4生化实验....................3
1.2.1.5PCR鉴定....................3
1.2.2药敏试验..............4
1.2.2.1菌悬液的制备..............4
1.2.2.2MIC测定..............4
2 结果与分析.4
2.1 细菌的分离鉴定......4
2．1.1 病料的分离及初步鉴定..4
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