高脂日粮对小鼠子宫发育的影响研究
毕业论文关键词：高脂日粮；肥胖；子宫；腺体；相关蛋白
Effects of high - fat diet on uterine development of miceAbstract: High fat diet can induce mouse obesity, causing animal liver fat and the body's metabolicdisorders, and cause the body's oxidative stress as well as other hazards, but effects of the high-fat diet onthe female animal uterus and other reproductive organs development as well as their related mechanismsare less studied currently. Therefore, the further study of the effects of high energy feed on uterinedevelopment in female mice is of great significance not only to study the mechanism of reproductivesystem development, but also to provide new methods and ideas for solving various problems caused byobesity. In this study, 10 female ICR mice were randomly pided into two groups. The obese mice modelwas induced by long-term feeding of experimental group (T) mice with high fat diet, the control group (C)was fed with regular diet. The effects of high-fat diet on the uterus development of mice were investigatedby hematoxylin and eosin (H&E) Staining and immunohistochemical (IHC) analysis, including somerelated protein such as PARP-1, PARG and Caspase-3. The results showed that the daily gain of thehigh-fat group was significantly higher than that of the control group, p < 0.05; the adult body weight of thehigh fat group was significantly higher than that of the control group, p < 0.05; the liver slices of the highfat group showed fatty liver symptoms. The levels of total Cholesterol (TC), triglyceride (TG) and lowdensity lipoprotein (LDL-C) were significantly higher than those in the control group (p < 0.05). What’smore, uterine glands are more sparse than the control group, the gland cavity is narrow, the shape ofirregular oval, there part of the absence of glandular area, and high fat group uterine slice of the number ofglands is significantly less than the control group, p < 0.05. In addition, PARP-1, PARG and caspase-3 wereexpressed in different degrees in the mouse uterus, but the expression of PARP-1, PARG and caspase-3 wasnot obvious in the high-fat group and the control group but needs further quantitative studies.
Key words: high-fat diet; obesity; uterus; glands; related proteins
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