摘要：目的：研究紫苏多糖对过氧化氢（H2O2）诱导的人肝细胞（LO2)损伤的保护作用及其可能的机制。方法：使用H2O2作用LO2细胞建立氧化损伤模型，Alamar blue 法检测细胞活力被用于观察紫苏多糖的保护作用；丙二醛( MDA) 含量、超氧化物歧化酶( SOD) 活性、过氧化氢酶(CAT)活力和谷胱甘肽（GSH）含量被用于检测其抗氧化活性及可能的机制。结果：紫苏多糖（50、100、200、400、800、1000μg/mL）可以减轻H2O2对人LO2细胞的氧化损伤，Alamar blue 检测紫苏多糖给药组，LO2细胞存活率增加；酶标法检测结果显示，紫苏多糖可以降低MDA含量，提高SOD和CAT活性以及GSH含量，且呈剂量关系。结论：LO2细胞经（0-1000μg/mL）紫苏多糖预处理后，可以缓解H2O2 造成的氧化损伤，维持正常的生理形态。51848

毕业论文关键词：紫苏多糖、H2O2、LO2细胞、氧化损伤

Protective Effects of Polysaccharides from Perilla on LO2 Cells Against Oxidative Damage Induced by Hydrogen Peroxide

Abstract: Objective The protection of polysaccharides from Perilla on LO2 cells injury induced by hydrogen peroxide and the possible underlying mechanism are investigated. Methods The injury model of LO2 cells were established by using H2O2 and the protective effects of Perilla were assessed by cell viability using alamar blue method. Results Perilla polysaccharides(50、100、200、400、800、1000μg/mL) significantly attenuated H2O2-induced decrease of cell viability. The survival rate of LO2 cells was increased by Perilla polysaccharides. The levels of MDA were significantly decreased, whereas the content of GSH and the activities of CAT and SOD were significantly increased by Perilla polysaccharides after H2O2-induced oxidative damage in a dose dependent manner. Conclusion Perilla polysaccharides can effectively attenuate H2O2-induced oxidative damage at the dose of 0-1000μg/mL and maintain the normal physiology of the cells.

Keywords: Perilla polysaccharides; H2O2; LO2 cells; oxidative damage 

目 录

前言 1

1.材料与仪器 1

1.1供试材料 1

1.2实验试剂 1

1.3主要仪器 2

1.4实验方法 2

1.4.1 细胞培养 2

1.4.2 H2O2诱导LO2细胞损伤模型的建立 2

1.4.3 紫苏多糖的给药浓度范围确定 2

1.4.4 紫苏多糖对H2O2损伤的LO2细胞的保护作用 2

1.4.5细胞MDA和GSH含量以及SOD和CAT活性的检测 3

2  结果与分析 3

2.1 不同浓度H2O2对LO2细胞活力的影响 3

2.2 紫苏多糖对LO2细胞的毒性作用 4

2.3 紫苏多糖对H2O2损伤的LO2细胞活力的影响 4

2.4  紫苏多糖对H2O2诱导的LO2细胞中SOD活性的影响 4

2.5  紫苏多糖对H2O2损伤的LO2细胞中MDA含量的影响 5

2.6  紫苏多糖对H2O2诱导的LO2细胞中CAT的活性的影响 5

2.7  紫苏多糖对H2O2诱导的LO2细胞中GSH含量的影响 6

3  讨论 6

致谢 7

参考文献 7

前言