绞股蓝多糖的结构测定和活性研究
Structure and Activity of Gynostemma
Abstract: We use Gynostemma pentaphyllum as raw material in this experiment, Gynostemma pentaphyllum polysaccharides (GPP) was prepared, and its structure and activity were studied. Using alkaline extraction method extracts the active polysaccharides from Gynostemma pentaphyllum.Using the Sevag method to take off the protein, hydrogen peroxide to pigment, column chromatography separation of DEAE-32 column chromatography separation further, then using S400 molecular sieve column chromatography separation, then get the pure Gynostemma pentaphyllum polysaccharides whose molecular weight was about 6.7KD.With the purified Gynostemma pentaphyllum polysaccharides for monosaccharide composition analysis, determination of the composition of rhamnose, arabinose, glucose and galactose, and the composition proportion is Rhamnose: arabinose: glucose: galactose: mannose =1: 2.63: 7.88: 4.27. Through biological methods hydroxyl radicals and superoxide radicals to its antioxidant and anti-inflammatory activity, indicating GPP has antioxidant and anti-inflammatory activity.
.Keywords: Gynostemma pentaphyllum, structure, antioxidant, anti-inflammatory
目 录
1 研究背景 1
1.1绞股蓝多糖的简介及来源 1
1.2 绞股蓝多糖的结构特点 1
1.3 绞股蓝多糖的生理活性 2
1.3.1 免疫调节 2
1.3.2 抗肿瘤 3
1.3.3 抗氧化 3
1.3.4 降血糖 3
1.3.5 抗肝损伤 4
1.4 绞股蓝多糖的国内外研究概况 4
1.5 本课题的研究意义及研究内容 5
1.5.1 研究意义 5
1.5.2 研究内容 5
2 实验材料及方法 6
2.1 实验材料 6
2.1.1 原料 6
2.1.2 主要试剂 6
2.1.3 主要仪器 7
2.2 实验方法 7
2.2.1葡萄糖标准曲线的建立 7
2.2.2 绞股蓝多糖样品的制备 8
2.2.3 绞股蓝多糖分子量测定及纯度研究 8
2.2.4 绞股蓝多糖结构测定 9
2.2.5 绞股蓝多糖活性研究 10
3 实验结果与讨论 11
3.1 葡萄糖标准曲线绘制结果 11
3.2 绞股蓝多糖分子量及纯度测定结果 12
3.2.1 绞股蓝多糖分子量测定结果 12
3.2.2 绞股蓝多糖纯度测定结果 13