Dd多糖的提取与纯化工艺研究
Study on the extraction and purification of polysaccharides
Abstract: Objective To study on the extraction and purification of polysaccharide. Methods Arrange the extraction and alcohol sink of Dd polysaccharide by orthogonal test, determinate Dd polysaccharide by the determination methodology verified, explore the optimum process conditions of water extraction and alcohol sink through the analysis of variance, and verify whether process is stable and feasible or not. Results The optimum process conditions for water extraction is extracting a material with 20 times of water 3 times, extract 2 hours every time. The optimum process conditions for alcohol sink precipitate a material with 4 times of liquid 4 hours in the condition concentration of alcohol sink is 50％. Conclusion The explored determination methodology of Dd polysaccharide is simple and practical, it saves cost and suits production industrialization.the explored optimum conditions of water extraction and alcohol sink is stable, the quality of polysaccharide is reliable.
Key words：polysaccharide, extraction, purification, determination , process research
目录
1绪论 1
1.1 Dd多糖的简介 1
1.2 文献综述 1
1.2.1 Dd多糖的国内外研究 1
1.2.2 Dd多糖的活性及作用机制研究进展 2
1.2.3 Dd多糖在食品工业中的应用 3
2 Dd多糖的方法学研究 5
2.1 实验与试剂 5
2.1.1实验仪器 5
2.1.2 药品与试剂 5
2.2 实验方法与结果 5
2.2.1含量测定方法的选择 5
2.2.2含量测定方法具体操作及样品溶液的制备 6
2.2.3 Dd多糖分析方法的验证 6
2.3小结 11
3.1 仪器与试剂 12
3.1.1实验仪器 12
3.1.2药品与试剂 12
3.1.3 数据处理软件 12
3.2 试验方法与结果 12
3.2.1 Dd多糖提取方法的选择 12
3.2.2水提醇沉具体操作 13
3.3水提与醇沉的正交试验设计 13
3.3.1水提工艺的正交试验设计 14
3.3.2 醇沉工艺的正交试验设计 16
3.4粗糖的纯化与精制[28] 19
3.5 小结 20
4 工艺验证 21
4.1水提工艺验证 21
4.1.1 仪器与试剂 21
4.1.2 实验内容 21
4.2 醇沉工艺验证 22
4.2.1 仪器与试剂 22
4.2.2 实验内容 23
4.3 小结 24
5 全文总结 25
致 谢 26
参考文献 27
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