超高效液相色谱-串联质谱法研究吡唑醚菌酯在田水和土壤中的残留和消解动态
关键词 吡唑醚菌酯 高效液相色谱-质谱 稻田水 土壤 残留 动态消解
毕业论文设计说明书外文摘要
Title Final residue and dissipation dynamics of pyraclostrobin in paddy water and soil by UPLC-MS
Abstract
【Objective】 The final residues and dynamic degradation of pyraclostrobin in paddy water and soil was researched by UPLC-MS/MS.【Method】The paddy water was extracted by dichloromethane and then concentrated before UPLC-MS/MS detection; The soil was extracted with acetonitrile by ultrasonic-assisted method, purified by primary secondary amine (PSA),and then detected by UPLC-MS/MS, with external standard method for quantitative.【Results】 The linearity of pyraclostrobin in the concentration of 0.01~1.0 mg/L was acceptable; within the range of 0.01-1.0 mg/L, the average recovery of field water and soil were 94.2%~106.9% and 78.4%-87.1%,respectively.The relative standard deviation (RSD) were 2.82%-6.53% and 1.17%~ 3.55%.The detection limit of the instrument was 0.1ug/L.In constant (100g a.i./ha) and high (150 g a.i./ha) medication, final residues of pyraclostrobin in paddy soils of Fujian and Nanjing both were less than 0.08 mg/kg; degradation dynamics curve conforms to the first-order kinetic equation and the pesticide residual in paddy water and soil decreased with the time prolongation. 【Conclusion】The research provides a fast and efficient analysis of residues and dynamic degradation of pyraclostrobin, and could de regarded as the guidance of safety and reasonable use of pyraclostrobin in agricultural production and provides the scientific basis for reducing the pollution to the environment.
Keywords pyraclostrobin; LC–MS/MS; paddy water; soil; residues; dissipation
目 录
1 绪论 1
1.1 吡唑醚菌酯 1
1.1.1 吡唑醚菌酯杀菌机理 1
1.1.2 吡唑醚菌酯的基本理化性质 1
1.2 高效液相色谱串联质谱法LC–MS/MS 2
1.2.1 高效液相色谱分析 2
1.2.2 质谱分析 3
1.2.3 液相色谱-质谱联用技术 4
1. 3 吡唑醚菌酯的残留分析 4
1.3.1 吡唑醚菌酯的残留分析研究现状 4
1.3.2 液质串联分析吡唑醚菌酯 5
2 实验过程 5
2.1 实验材料与方法 5
2.1.1 仪器与试剂 5
2.1.2 仪器条件 5
2.2 田间试验 6
2.2.1 试验地点 6