山羊胃肠道组织中T1R3基因的表达差异研究
毕业论文关键词：山羊；T1R3；实时定量PCR；差异分析
The study on Difference Expression of T1R3 Gene in Goat Gastrointestinal Tract
Abstract: In this paper, through the relative quantification PCR, we made research and analysis of expression of T1R3 gene in the xuhuai goat gastrointestinal tract. Provide a theoretical basis for solving the problem of amino acid metabolism and requirements. By means of gathering goat gastrointestinal tract, containing rumen, omasum, reticulum, abomasum, rectum, duodenum, cecum, jejunum, colon and ileum. After that, it is necessary to extract the Total RNA, keep an account and check sample potency and purity. Then, by means of the real-time quantitative PCR to deal with cDNA. In this study, the expression of the T1R3 mRNA in the gastrointestinal tract of goat show significant differences. The research report was as follows, ileum (1.26), duodena (1.00), rectum (0.55), Jejunum (0.38), cecuml (0.025), colonic (0.0035), abomasum (0.002), omasum (0.00196), rumens (0.0008), and reticulum (0.0005). In this research, we can found that the T1R3 gene express in each tissue of goat gastrointestinal tract. The tissues which the mRNA relative expression of T1R3 expressed the highest level was ileum, duodena and rectum, but the low quantity expressed in stomach tissue.
Key words: goat; T1R3; real-time PCR; variation analysis
目 录
前言 1
1 实验过程、材料和方法 2
1.1实验过程 2
1.2 实验材料 2
1.2.1实验样品来源 2
1.2.2实验仪器 2
1.2.3 实验试剂 3
1.3实验方法 3
1.3.1提取的各组织的总RNA 3
1.3.2提取的总RNA的质量检测 3
1.3.3样品RNA浓度和纯度的数据 3
1.3.4 反转录合成CDNA 4
1.3.5荧光实时定量PCR 5
2 结果与分析 5
2.1 PCR定量结果数据 5
2.2 目的基因和内参的扩增 6
2.3 PCR数据处理 8
2.4 T1R3基因差异性表达结果 8
3讨论 9
致谢 10
参考文献 11
前言
山羊出现在中国人民的生活和饮食上已经有几千年的历史，对于中国人民的生活产生了深刻的影响，人们通过饲养山羊获得经济及生活食用来源，一步步的推动了中华民族的前进。自从1978年以后，山羊的饲养在中国逐渐扩大，数量不断增多，分布越来越广，而且通过实践积累，人们对于山羊的饲养经验阅历都丰富起来，很好的带动了地方及全国的经济效益，提高了整个国家人民的生活水平状况。我们通过对山羊机体各组织的研究分析，希望从基因水平调控山羊的性能，从而为国家人们带来更加美好的生活。