竹荪发酵产抗氧化活性成分的研究
毕业论文关键词：竹荪；发酵；提取；定性检测；抗氧化性
Study on active antioxidant ingredients which are fermented by dictyophora
Abstract: We found the most suitable fermentation conditions for dictyophora by fermenting strains, and that the condition of 8 L/min in ventilation, 100rpm, 10% in inoculum and 26oC are the best. Then we fermented some dictyophora strains and Freeze-dried them by gauze, after which we continued to ultrasonic extract the production with different concentrations of alcohol, and boiled extracted it with different concentrations of water. Finally qualitative tested the supernatant which had been centrifuged and analysed the antioxidant ingredients. The extraction of dictyophora has the ability to remove DPPH, which is quick (60 min) and powerful(IC50 is 0.01154mg/mL). So to speak, it contains active antioxidant ingredients and is worth further studying.
Key Words: Dictyophora; fermentation; extraction; qualitative detection; antioxidant
目录
1 绪论 1
1.1 竹荪特性研究概况 1
1.1.1 降血压、血脂作用 1
1.1.2 抗辐射、调节免疫作用 2
1.1.3 抗肿瘤作用 2
1.1.4 抗氧化作用 2
1.1.5 细胞的凝集作用 2
1.1.6 抑菌作用 2
1.2 定性检测实验的研究概况 3
1.2.1定性分析诠释 3
1.2.2分类 4
1.2.3分析方法 4
1.2.4操作方式 4
1.2.5灵敏度 4
1.2.6干扰 5
1.2.7分析要求 5
1.2.8分析步骤 6
1.3 抗氧化实验的研究概况 7
1.3.1 化学评价法 8
1.3.2 生物活性评价法 9
1.4研究目的和意义 9
1.5 主要研究内容 9
2 实验材料和方法 10
2.1实验材料 10
2.1.1原料与试剂 10
2.1.2 仪器与设备 11
2.2 实验方法 11
2.2.1菌丝体培养 11
2.2.2发酵罐培养 11
2.2.3菌丝体和发酵液的干燥 12
2.2.4菌丝体和发酵液的提取 12
2.2.5生物碱的定性检验： 12
2.2.6氨基酸、肽和蛋白质检验： 12
2.4.3有机酸的定性检验： 12
2.2.7酚类化合物和鞣质的定性检验： 13
2.2.8甾体的定性检验： 13
2.2.9黄酮体的定性检验： 13
2.2.10蒽醌类的定性检验： 13
2.2.11糖类的定性检测 14
2.2.12 DPPH•自由基储备液的制备 14
2.2.13 DPPH•自由基溶液最大吸收波长的确定 14
2.2.14 DPPH•自由基标准曲线的建立 15