阿维菌素在农田土壤中的残留和降解特性研究
关键词：阿文菌素；高效液相色谱法；残留；土壤
Study on the Residue Detection and Degradation of Abamectin in Agricultural Soil
ABSTRACT: Abamectin is the most commonly used Biological pesticides on prevention and cure of main pests and diseases of crops, such as rice leaf roller disease. It has been widely used in China and other countries. This study established a method of pre-treatment and instrumental analysis of abamectin through the experiments, and discussed its degradation in soil. Acetone was used to extract the residue of abamectin in soil under the condition of oscillation. Established Waters Alliance HPLC as the method of instrumental analysis, choosing XBridgeTM C18 column(4.6mm×250mm，5μm) as the column. Used the way of constant current elution, and the methanol:water as the mobile phase. UV detector was chosen for determining the content of abamectin at the wavelength of 245nm. The result indicated that the lowest detection limit of abamectin in the soil sample was 0.016mg/L, and the method had a good linearity with peak area within 0.016mg/L-323.3mg/L. The linear regression equation was Y=35170X+, and its correlation coefficient was 0.9999. The Recoveries were 88.05% to 99.97% when the concentration of the abamectin standard ranged from 0.1 to 10mg/kg, and the coefficients of variation were 0.52% to 6.39%. The degradation half-life of abamectin was 5.7d. The data showed that the method met the demands of pesticide residue analysis.
Key words: abamectin; HPLC; residue; soil
目录
第一章 绪论部分 - 1 -
1.1 阿文菌素简介 - 1 -
1.1.1 阿文菌素的制备方法 - 1 -
1.1.2 阿文菌素的作用方式及特点 - 2 -
1.2 阿文菌素的分析方法 - 2 -
1.2.1 固相萃取-高效液相色谱法 - 2 -
1.2.2 柱前衍生-高效液相色谱法 - 2 -
1.2.3 超高效液相色谱-串联质谱法 - 3 -
1.2.4 反相高效液相色谱法 - 3 -
1.2.5 高效液相色谱-荧光检测法（HPLC-FLD） - 3 -
1.2.6 高效液相色谱-紫外检测法（HPLC-UV） - 4 -
1.2.7 一阶导数光谱法 - 4 -
1.2.8 酶联免疫法（ELISA） - 4 -
1.3 阿文菌素目前在各环境中的残留情况 - 5 -
1.3.1 阿文菌素在水样品中的残留 - 5 -
1.3.2 阿文菌素在植物样品中的残留 - 6 -
1.3.3 阿文菌素在土壤样品中的残留 - 6 -
1.3.4 阿文菌素在动物粪便样品中的残留 - 7 -
1.4 阿文菌素在环境中的降解情况 - 7 -
下一篇：取代苯异腈与脂肪胺的加成反应