不同提取方法对茶树花多糖性质的影响研究
毕业论文关键词：茶树花；多糖；提取方法；理化性质；抗氧化活性
Effects of Different Extraction Methods on the Polysaccharides from the Flower of Camellia sinensis
Abstract：Tea flower is a genital organ of tea (Camellia sinensis), rich in a variety of nutrients and active substances, including tea tree polysaccharides, tea polyphenols, tea saponins, flavonoids and tea tree essential oil, which tea tree polysaccharide has a significant anti Radiation, anti-cancer, hypolipidemic and so on. Tea tree flowers as a by-product utilization is far less than tea, which resulted in a lot of waste of resources. In view of this phenomenon, this paper studied the effects of different extraction methods on the properties of polysaccharides from tea tree. The polysaccharides were obtained by means of hot water extraction, enzyme extraction, ethanol refluxing and ultrasonic assisted extraction. The physicochemical properties and antioxidant activities of polysaccharides extracted from different methods were compared and analyzed by high performance liquid chromatography so the polysaccharide composition of tea polysaccharide was tested.
Key words: tea flower; polysaccharide; extraction method;physical and chemical properties; antioxidative activity
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法 2
1．1 茶树花多糖的提取方法 2
1．1．1 热水浸提法 2
1．1．2 乙醇回流法 2
1．1．3 酶解提取法 2
1．1．4 超声波辅助提取法 2
1．1．5 茶树花多糖得率 2
1．2 茶树花粗多糖理化性质的测定 2
1．2．1 总糖含量的测定 2
1．2．2 蛋白质含量的测定 3
1．2．3 多酚含量测定 3
1．2．4 糖醛酸含量测定 3
1．2．5 硫酸基含量测定 3
1．3 抗氧化活性测定 4
1．3．1 清除DPPH自由基 4
1．3．2 清除羟基自由基 4
1．3．3 清除超氧阴离子自由基 4
1．3．4 清除ABTS自由基 4
1．3．5 金属离子螯合能力测定 5
1．4 茶树花多糖的单糖组成分析 5
1．4．1 多糖样品的水解 5
1．4．2 PMP柱前衍生化 5
1．4．3 高效液相色谱（HPLC）法检测 5
1．4．4 单糖混标的配置 5
2 结果与分析 6
2．1 茶树花多糖得率 6
2．2 茶树花理化性质 6
2．3 抗氧化活性 7
2．3．1 清除DPPH自由基 7
2．3．2 清除羟基自由基 7
2．3．3 清除超氧阴离子自由基 8
2．3．4 清除ABTS自由基 8