高分辨多级质谱识别食用油中的甘油三酯
毕业论文关键词：甘油三酯；大气压电离，多级质谱，食用油
Use High-resolution multi-stages mass spectrometry to identify triglycerides in the oil
Abstract: The essay studies about the method of multi-stages mass spectrometry to analysis triglyceride in oil. The solvent is isopropyl alcohol, chloroform is added to the oil, and multi-stages mass spectrometry is used for test. Using a better APCI source, which feeding velocity is 1200 mu L/h, drying temperature is 180 ℃, and APCI current is 5000 nA, to measure cooking oil by high resolution mass spectrometry. Measure the highest peak’s secondary mass spectrometry, determine the first one and third fatty acids in triglycerides. Analyze fracture rules of triolein as a prototype, obtain two kinds of fatty acid according to the multi-stages mass spectrometry feedback information, and deduce the structure of triglyceride in the oil. Use this method the main types of triglycerides are measured in the corn oil, soybean oil, sunflower oil, olive oil, and lard. 9 kinds of triglycerides are detected in the corn oil, 9 kinds of triglycerides detected in soybean oil , 4 kinds of triglyceride are detect in sunflower oil, 7 kinds of triglycerides are detected in the olive oil, and 8 kinds of triglycerides are detected in pigs. This method is easy and fast, and a lot of time of handling or separation can be saved.
Key Words：Triglycerides；Mass spectrometry；APCI；cooking oil
目录
1.前言 5
1.1 选题意义 5
1.2 文献综述 6
1.2.1质谱 6
1.2.1.1傅立叶变换质谱 6
1.2.1.2飞行时间质谱 6
1.2.1.3离子阱质谱 7
1.2.4.1轨道阱质谱 7
1.2.4.2磁质谱 8
1.2.2质谱联用 8
1.2.2.1电喷雾串联质谱 8
1.2.2.2电喷雾化学电离与飞行时间质谱联用 9
1.2.2.3基质辅助激光解析电离与飞行时间质谱联用 10
1.2.2.4超临界流体色谱和质谱联用 10
1.2.3小结 11
2.仪器与试剂 12
2.1 仪器： 12
2.1.1 SolariX 70 FT-MS测样流程 12
2.2试剂： 12
2.3 样品来源 12
3.实验内容 13
3.1样品处理 13
3.2实验方法 13
4.结果与讨论 13
4.1样品溶液配比 13
4.2离子源的选择 14
4.2.1小结 14
4.3质谱测定甘油三酯结果 15
4.3.1标样的测定 15
4.3.2玉米油中甘油三酯的测定 16
4.3.3豆油中甘油三酯的测定 17