四环素对蓝藻的急性毒性研究+实验讨论
关键词： 四环素、铜绿微囊藻、急性毒性、抑制、降解4209
Acute toxicity of tetracycline on Microcystis aeruginosa
Abstract: Tetracycline antibiotics are widely used in the world. This is one of the largest types of antibiotics, which can remain in the environment and bring potential environmental risks. In this study, the eco-toxicity of tetracyclines on cyanobacteria Microcystis aeruginosa was studied. Growth inhibition of this kind of algae exposure to tetracycline was measured. pH values, protein content, were also studied to determine the acute toxicity of tetracycline on M. aeruginosa. The results showed that at low concentration 1 mg/L tetracycline can stimulate the growth of M. aeruginosa, and in prolonged exposure to high concentrations of tetracycline, M. aeruginosa growth was suppressed. This paper also detected the degradation of tetracycline in M. aeruginosa culture medium by high performance liquid chromatography (HPLC). The results showed that the reaction is a first-order kinetics reaction, Ln (tetracycline concentration) and the reaction time showed linear relationship. And discussed the future research directions, aimed to provide valuable reference for the ecological risk assessment of the antibiotics.
KeyWords: Tetracycline; Microcystis aeruginosa; Acute toxicity; Inhibit; Degradation
目录
1. 引言（绪论） 1
1.1 抗生素的污染现状 1
1.2 四环素类抗生素的污染现状 2
1.3 四环素类抗生素的生态毒性 3
1.3.1 四环素类抗生素对微生物的生态毒性 3
1.3.2 四环素类抗生素对植物的生态毒性 4
1.3.3 四环素类抗生素对动物的生态毒性 4
1.4 四环素的理化性质和药理毒理 5
1.4.1 四环素的理化性质 5
1.4.2 四环素的药理毒理 5
1.5 蓝藻、铜绿微囊藻 6
1.5.1 蓝藻概述 6
1.5.2 铜绿微囊藻概述 6
1.5.3 藻类水华 6
1.6 微囊藻毒素的污染现状 7
1.6.1 微囊藻毒素的污染现状 7
1.6.2 天然及水库水体的微囊藻毒素污染 7
1.6.3 饮用水的微囊藻毒素污染 8
1.7 课题研究的意义和目的 8
1.8 课题研究的内容 8
2. 实验条件及预实验 9
2.1 实验条件 9
2.1.1 实验仪器 9
2.1.2 实验材料 9
2.1.3 实验药品 11
2.2 预实验 11
2.2.1 BG11培养液的配置 11
2.2.2 高压蒸气灭菌 12
2.2.3 铜绿微囊藻的接种 12
2.2.4 铜绿微囊藻的培养 12
2.3 实验方案论证 12
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