金霉素对蓝藻的急性毒性研究
关键词：金霉素；铜绿微囊藻；生长趋势；细胞含量；蛋白含量4065
Acute Aquatic Toxicity of Chlortetracycline
on Microcysis aeruginosa
Abstract: Chlortetracycline is regarded as one of widespread antibiotics currently.It threatens the safety of aquatic environment on account of antibiotics’ existing in aquatic systems. In this study, Microcysis aeruginosa is model organism. Cell content, growth tendency and protein content were selected as experimental indicators to assess the toxic effects of chlortetracycline on aquatic life. The results show that chlortetracycline may promote growth of Microcysis aeruginosa at low concentration in a short term, but chlortetracycline will promote growth of Microcysis aeruginosa in a long term. And the inhibition rate towards Microcysis aeruginosa raises together with the concentration of chlortetracycline, for instance, the biggest inhibition rate is 7.1927 % at 1 ppm while the biggest inhibition rate is 52.5489% at 15 ppm.Through the present study, the effects of acute toxicity on Microcystis aeruginosa could be understood, and it has a significant meaning to use antibiotics rationally.
Keywords: chlortetracycline；Microcysis aeruginosa；growth tendency；cell content；protein content
目录
1.引言 1
1.1选题背景 1
1.2 金霉素的物理化学性质 2
1.3 金霉素的基本情况 3
1.4 铜绿微囊藻的研究进展 4
2.研究方案与方法 7
2.1 研究目标和内容 7
2.2 实验铜绿微囊藻的培养 7
2.3 实验方案 9
3.实验部分 11
3.1 藻体生物量与抑制率测定 11
3.1.1 藻体生物量测定 11
3.1.2 藻体抑制率测定 12
3.2 蛋白含量测定 12
3.3 pH的测定 12
4.结果与讨论 13
4.1 显微镜计数与吸光度之间的线性关系 13
4.2 生长曲线与抑制率 13
4.3 蛋白含量 16
4.4 pH 18
4.5 结论 19
致谢 20
参考文献 21
1 引言
金霉素(Chlortetracycline，CTC)，又名氯四环素，是由美国氰氨公司于1949年开发的四环素类广谱抗生素，对革兰阳性菌，阴性菌，立克次体和大型病毒有广泛的抑制能力。金霉素纯品为金黄色粉末，无臭苦，熔点168°C～169°C。由于金霉素分子中含有酚羟基，烯醇和二甲氨基，属于酸碱两性物质，易溶于酸性或碱性溶液。
金霉素是一种高效广谱抗生素，对多种病原菌有较强的抑制作用，对革兰氏阳性球菌，特别是葡萄球菌、肺炎球菌有效，常用于动物各DNA种传染性疾病的治疗。但其在动物肉、奶、蛋等食品中的残留严重威胁人体健康，能引起再生障碍性贫血和粒状白细胞缺乏症等疾病，低浓度的药物残留还会诱发致病菌的耐药性。
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